US 11,718,643 B2
Multimodal anion exchange matrices
Jean-Luc Maloisel, Uppsala (SE); Gustav Rodrigo, Uppsala (SE); Bjorn Noren, Uppsala (SE); and Virendra Kumbhar, Gurgaon (IN)
Assigned to Cytiva BioProcess R&D AB, Uppsala (SE)
Appl. No. 14/441,129
Filed by GE HEALTHCARE BIOPROCESS R&D AB, Uppsala (SE)
PCT Filed Nov. 11, 2013, PCT No. PCT/SE2013/051325
§ 371(c)(1), (2) Date May 6, 2015,
PCT Pub. No. WO2014/077762, PCT Pub. Date May 22, 2014.
Claims priority of application No. 1251286-9 (SE), filed on Nov. 13, 2012.
Prior Publication US 2015/0299248 A1, Oct. 22, 2015
This patent is subject to a terminal disclaimer.
Int. Cl. B01D 15/32 (2006.01); B01D 15/36 (2006.01); B01D 15/38 (2006.01); B01J 20/26 (2006.01); B01J 20/28 (2006.01); B01J 20/286 (2006.01); B01J 20/289 (2006.01); B01J 20/32 (2006.01); B01J 41/20 (2006.01); C07K 1/16 (2006.01); C07K 1/36 (2006.01); C07K 16/00 (2006.01); C07K 16/06 (2006.01); G01N 33/544 (2006.01)
CPC C07K 1/165 (2013.01) [B01D 15/327 (2013.01); B01D 15/363 (2013.01); B01D 15/3847 (2013.01); B01J 20/265 (2013.01); B01J 20/286 (2013.01); B01J 20/289 (2013.01); B01J 20/28016 (2013.01); B01J 20/3204 (2013.01); B01J 20/3208 (2013.01); B01J 20/3219 (2013.01); B01J 20/3251 (2013.01); B01J 20/3253 (2013.01); B01J 20/3255 (2013.01); B01J 20/3285 (2013.01); B01J 41/20 (2013.01); C07K 1/36 (2013.01); C07K 16/00 (2013.01); C07K 16/065 (2013.01); G01N 33/544 (2013.01); B01D 15/3809 (2013.01); B01D 15/3885 (2013.01); B01J 2220/52 (2013.01); B01J 2220/80 (2013.01)] 15 Claims
OG exemplary drawing
 
1. A separation matrix comprising a plurality of separation ligands defined by formula (I)
R1-L1-N(R3)-L2-R2  (I)
immobilized on a support via the amine group of formula (I),
wherein R1 is a hydroxyethyl group or a five- or six-membered, substituted or non-substituted ring structure;
L1 is either a methylene group or a covalent bond;
R2 is a five- or six-membered, substituted or non-substituted ring structure;
L2 is either a methylene group or a covalent bond; and
R3 is a methyl group;
wherein said ligands in free form have an octanol-water distribution coefficient (lg P) of 1.8-5;
wherein if R1 is a hydroxyethyl group, L1 is a covalent bond and R2 is a substituted aromatic ring structure or a substituted or non-substituted aliphatic ring structure; and
wherein the separation ligands are defined by formula (IV) or (V):

OG Complex Work Unit Chemistry