CPC C07H 21/04 (2013.01) [C07H 19/06 (2013.01); C07H 19/16 (2013.01); C07K 14/36 (2013.01); C09B 23/083 (2013.01); C09B 69/105 (2013.01); C12N 9/10 (2013.01); C12Q 1/6869 (2013.01); C12Q 1/6874 (2013.01); G01N 33/582 (2013.01); G01N 33/583 (2013.01); C12Q 2523/313 (2013.01); C12Q 2535/113 (2013.01); C12Q 2563/107 (2013.01); C12Q 2563/131 (2013.01)] | 13 Claims |
1. A method of making a labeled nucleotide analog comprising:
(a) providing an avidin protein having four subunits, each subunit comprising one biotin binding site;
(b) adding a nucleotide component, the nucleotide component comprising one or more phospholinked nucleotide moieties covalently connected to a bis-biotin moiety, whereby the bis-biotin moiety binds to two of the four subunits on the avidin protein, and
(c) adding a dye component comprising one or more dye moieties covalently connected to a bis biotin moiety, whereby this bis-biotin moiety binds to the other two subunits on the avidin protein thereby forming a nucleotide analog.
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