CPC B01L 3/502761 (2013.01) [B01L 3/50851 (2013.01); B01L 3/502738 (2013.01); B01L 7/52 (2013.01); B01L 7/525 (2013.01); C12Q 1/686 (2013.01); G01N 35/0098 (2013.01); B01L 3/50855 (2013.01); B01L 2200/0668 (2013.01); B01L 2200/0684 (2013.01); B01L 2200/0689 (2013.01); B01L 2200/10 (2013.01); B01L 2300/044 (2013.01); B01L 2300/0627 (2013.01); B01L 2300/0681 (2013.01); B01L 2300/087 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0867 (2013.01); B01L 2300/0887 (2013.01); B01L 2300/168 (2013.01); B01L 2300/1822 (2013.01); B01L 2300/1827 (2013.01); B01L 2400/043 (2013.01); B01L 2400/0406 (2013.01); B01L 2400/0481 (2013.01); B01L 2400/0487 (2013.01); B01L 2400/0622 (2013.01); B01L 2400/0633 (2013.01); B01L 2400/0655 (2013.01); B01L 2400/0694 (2013.01)] | 11 Claims |
1. A system for automatically processing nucleic acid content of one or more samples in cooperation with a cartridge comprising a set of microfluidic pathways for performing amplification and detection of said nucleic acid content, the system comprising:
one or more process containers for capturing nucleic acid content from the one or more samples at a set of beads coupled with chemical entities for capturing said nucleic acid content;
a molecular diagnostic module comprising:
a cartridge location for positioning the cartridge within the molecular diagnostic module,
one or more heaters at the cartridge location for amplifying said nucleic acid content, and
an optical subsystem for detecting said nucleic acid content;
one or more storage containers for one or more reagents, the one or more reagents comprising a release solution for releasing at least a portion of the nucleic acid content from the set of beads, and reagents for amplifying one or more nucleic acids;
a fluid handling subsystem comprising an actuator and an aspiration and delivery component coupled to the actuator; and
a processor and a controller, the processor comprising a non-transitory computer-readable medium comprising instructions stored thereon, that when executed, cause the processor and controller to:
instruct the molecular diagnostic module to position the cartridge at the cartridge location,
instruct the fluid handling subsystem to execute a delivery mode with movement of the aspiration and delivery component of the fluid handling system between the one or more storage containers, and one or more inlets of the cartridge, thereby delivering said nucleic acid content into the cartridge,
instruct the one or more heaters to heat one or more of the set of microfluidic pathways of the cartridge, thereby performing amplification and detection of one or more nucleic acids, and
instruct the molecular diagnostic module to deliver the cartridge from the cartridge location.
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