US 11,717,824 B2
Methods of analyzing biological samples using a fluidic cartridge
Wendian Shi, Irvine, CA (US); and Yuzhe Ding, Irvine, CA (US)
Assigned to CytoChip Inc., Irvine, CA (US)
Filed by CytoChip, Inc., Irvine, CA (US)
Filed on Mar. 29, 2021, as Appl. No. 17/215,206.
Application 17/215,206 is a continuation of application No. 15/989,020, filed on May 24, 2018, granted, now 10,967,374.
Application 15/989,020 is a continuation of application No. 15/176,729, filed on Jun. 8, 2016, granted, now 10,022,720, issued on Jul. 17, 2018.
Claims priority of provisional application 62/174,776, filed on Jun. 12, 2015.
Prior Publication US 2021/0213445 A1, Jul. 15, 2021
Int. Cl. B01L 3/00 (2006.01); A61B 5/15 (2006.01); A61B 10/00 (2006.01)
CPC B01L 3/50273 (2013.01) [A61B 5/150022 (2013.01); A61B 10/0096 (2013.01); B01L 3/502715 (2013.01); B01L 3/502723 (2013.01); B01L 3/502738 (2013.01); B01L 2200/141 (2013.01); B01L 2200/143 (2013.01); B01L 2300/0627 (2013.01); B01L 2300/0645 (2013.01); B01L 2300/0681 (2013.01); B01L 2300/087 (2013.01); B01L 2300/0861 (2013.01); B01L 2300/0864 (2013.01); B01L 2300/0867 (2013.01); B01L 2300/0887 (2013.01); B01L 2300/14 (2013.01); B01L 2400/0406 (2013.01); B01L 2400/0409 (2013.01); B01L 2400/0457 (2013.01); B01L 2400/0487 (2013.01); B01L 2400/0688 (2013.01); B01L 2400/088 (2013.01)] 9 Claims
OG exemplary drawing
 
1. A method for analyzing a biological sample, comprising:
(a) receiving the biological sample into a cartridge device, wherein the cartridge device comprises:
a first fluidic unit including a first fluidic chamber and a first venting port, the first venting port being configured to apply a first pneumatic source to the first fluidic chamber;
a second fluidic unit including a second fluidic chamber and a second venting port, the second venting port being configured to apply a second pneumatic source to the second fluidic chamber; and
a fluidic connection between the first fluidic unit and the second fluidic unit;
(b) forming a fluid sample comprising at least a portion of the biological sample and particles in the first fluidic chamber;
(c) removing a portion of the fluid sample from the first fluidic chamber; and
(d) introducing a wash buffer into the first fluidic chamber such that at least a portion of the particles forms a fluid suspension with the wash buffer in the first fluidic chamber.