US 11,717,586 B2
Compositions and methods for reducing bioburden in chromatography
Nathan L. Mao, Cohoes, NY (US); Wenbin Qi, Carlsbad, CA (US); Bernhard Schilling, Hudson, NY (US); and Scott Carver, Wynantskill, NY (US)
Assigned to Regeneran Pharmaceuticals, Inc., Tarrytown, NY (US)
Filed by Regeneron Pharmaceuticals, Inc., Tarrytown, NY (US)
Filed on Jun. 24, 2022, as Appl. No. 17/848,936.
Application 17/153,205 is a division of application No. 15/882,845, filed on Jan. 29, 2018, granted, now 10,925,986, issued on Feb. 23, 2021.
Application 17/848,936 is a continuation of application No. 17/153,205, filed on Jan. 20, 2021, granted, now 11,369,707.
Claims priority of provisional application 62/452,140, filed on Jan. 30, 2017.
Prior Publication US 2022/0331468 A1, Oct. 20, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. A61L 2/18 (2006.01); B01D 15/38 (2006.01); G01N 30/50 (2006.01); B01D 15/20 (2006.01); A61L 2/28 (2006.01); G01N 30/88 (2006.01)
CPC A61L 2/18 (2013.01) [A61L 2/28 (2013.01); B01D 15/20 (2013.01); B01D 15/3809 (2013.01); G01N 30/50 (2013.01); G01N 2030/8831 (2013.01)] 14 Claims
OG exemplary drawing
 
1. A method for microbial bioburden reduction of a chromatography matrix, comprising: contacting a chromatography matrix with a composition comprising from about 0.1 M to about 0.5 M acetic acid, wherein the contacting step is performed for about 1 hour to about 4 hours, wherein the composition does not comprise an alcohol, guanidine hydrochloride, or urea, and the method does not comprise a step of contacting the chromatography matrix with a sodium hydroxide solution.