US 11,717,544 B2
Pairing probiotics and prebiotics, methods for growth and use, separately and in combination
Shayne Morris, Ogden, UT (US); and Michael Benson, Newton, UT (US)
Filed by Shayne Morris, Ogden, UT (US); and Michael Benson, Newton, UT (US)
Filed on Feb. 25, 2022, as Appl. No. 17/680,830.
Application 17/680,830 is a continuation of application No. 16/886,399, filed on May 28, 2020, granted, now 11,291,696.
Application 16/886,399 is a continuation in part of application No. 16/380,226, filed on Apr. 10, 2019, granted, now 11,168,299, issued on Nov. 9, 2021.
Claims priority of provisional application 62/655,935, filed on Apr. 11, 2018.
Claims priority of provisional application 62/854,490, filed on May 30, 2019.
Prior Publication US 2022/0175854 A1, Jun. 9, 2022
Int. Cl. A61K 35/742 (2015.01); A61K 35/744 (2015.01); A61K 9/19 (2006.01); C12N 1/20 (2006.01)
CPC A61K 35/742 (2013.01) [A61K 9/19 (2013.01); A61K 35/744 (2013.01); C12N 1/20 (2013.01)] 18 Claims
 
1. A method for growing a probiotic organism, comprising:
selecting at least one probiotic organism from the group consisting of Bacillus clausii, Lactobacillus casei, Akkermansia muciniphila, Eubacterium rectale, Lactococcus lactis, Lactobacillus bulgaricus, Lactobacillus buchneri, Pediococcus acidilactici, Bifidobacterium longum, Lactobacillus brevis, Lactobacillus delbrueckii subsp. bulgaricus, Propionibacterium shermanii, Butyricicoccus pullicaecorum, Lactobacillus acidophilus, Lactobacillus rhamnosus GG, Lactobacillus gasseri, Anaerobutyricum hallii, Bifidobacterium catenulatum, and Bifidobacterium pseudocatenulatum;
providing a growth broth for growing the probiotic organism, wherein the growth broth comprises 500-5000 mg of Bael fiber, 500-5000 mg of Fenugreek fiber, 500-5000 mg of quinoa sprout, 500-5000 mg of chia seed, 500-5000 mg of millet sprout, 500-5000 mg of buckwheat sprout, 500-5000 mg of N-acetyl glucosamine amino glycan, 50-500 mg of chondroitin, 500-5000 mg of mucin, 500-5000 mg of Chitosan oligosaccharide, 500-5000 mg of carrot fiber, 500-5000 mg of soluble corn fiber, 500-5000 mg of acemannan, 500-5000 mg of pea fiber, 50-1000 mg of Acacia catechu, 50-1000 mg of Pullulan, 50-1000 mg of Althaea officinalis (marshmallow root), and 500-5000 mg of camels milk;
inoculating the growth broth with the probiotic organism;
growing the probiotic organism in the growth broth;
harvesting the probiotic organism from the growth broth;
providing an agar spread plate media for growing the probiotic organism, wherein the agar spread plate media comprises 500-5000 mg of Bael fiber, 500-5000 mg of Fenugreek fiber, 500-5000 mg of quinoa sprout, 500-5000 mg of chia seed, 500-5000 mg of millet sprout, 500-5000 mg of buckwheat sprout, 500-5000 mg of N-acetyl glucosamine amino glycan, 50-500 mg of chondroitin, 500-5000 mg of mucin, 500-5000 mg of Chitosan oligosaccharide, 500-5000 mg of carrot fiber, 500-5000 mg of soluble corn fiber, 500-5000 mg of acemannan, 500-5000 mg of pea fiber, 50-1000 mg of Acacia catechu, 50-1000 mg of Pullulan, 50-1000 mg of Althaea officinalis (marshmallow root), and 500-5000 mg of camels milk;
inoculating the agar spread plate media with the probiotic organism harvested from the growth broth;
growing the probiotic organism on the agar spread plate media; and
harvesting the probiotic organism from the agar spread plate media.