US 11,717,542 B2
Using tumor-navigating Salmonella to modulate tumor metabolism
Wei Kong, Phoenix, AZ (US); Lingchen Fu, Tempe, AZ (US); Yixin Shi, Phoenix, AZ (US); and Bo Ning, Tempe, AZ (US)
Assigned to ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY, Scottsdale, AZ (US)
Appl. No. 17/433,225
Filed by ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY, Scottsdale, AZ (US)
PCT Filed Feb. 20, 2020, PCT No. PCT/US2020/019110
§ 371(c)(1), (2) Date Aug. 23, 2021,
PCT Pub. No. WO2020/172461, PCT Pub. Date Aug. 27, 2020.
Claims priority of provisional application 62/809,382, filed on Feb. 22, 2019.
Prior Publication US 2021/0386795 A1, Dec. 16, 2021
Int. Cl. A61K 35/74 (2015.01); A61P 35/00 (2006.01); C07K 14/255 (2006.01); C12N 1/20 (2006.01); C12N 9/12 (2006.01)
CPC A61K 35/74 (2013.01) [A61P 35/00 (2018.01); C07K 14/255 (2013.01); C12N 1/20 (2013.01); C12N 9/1205 (2013.01); C12Y 207/0104 (2013.01)] 15 Claims
 
1. A genetically modified tumoricidal Salmonella bacterium, wherein the bacterium is a self-eradicating, genetically modified χ3761 Salmonella bacterium comprising
ΔPmurA25::TT araC PBAD murA,
Δ(wcaM-wca)-8 ΔrelA198::araC PBAD laclTT,
Δ(araC PBAD)-18::P22 PR araBAD,
ΔpagP81::Plpp IpxE,
ΔendA1123 ΔPtar::Ptrc ΔlacO888 tar,
ΔPtsr::Ptrc ΔlacO888 tsr,
Δtrg,
ΔpvkA,
and ΔpykF, wherein the bacterium comprises a lysis vector pYA3681 expressing murA in the presence of arabinose, the lysis vector comprising pBR ori araC* PBAD SD-GTG asdA SD-GTG murA P22 PR anti-sense mRNA.