US 11,717,167 B2
In-vivo monitoring of cellular energetics with Raman spectroscopy
John P. Romfh, Palo Alto, CA (US); Daryoosh Vakhshoori, Cambridge, MA (US); John N. Kheir, Charlestown, MA (US); Peili Chen, Andover, MA (US); Brian Polizzotti, Swampscott, MA (US); Joshua Salvin, Sherborn, MA (US); and Alison Perry, Sevenoaks (GB)
Assigned to Pendar Technologies, LLC, Cambridge, MA (US); and Children's Medical Center Corporation, Boston, MA (US)
Filed by John P. Romfh, Palo Alto, CA (US); Daryoosh Vakhshoori, Cambridge, MA (US); John N. Kheir, Charlestown, MA (US); Peili Chen, Andover, MA (US); Brian Polizzotti, Swampscott, MA (US); Joshua Salvin, Sherborn, MA (US); and Alison Perry, Sevenoaks (GB)
Filed on Mar. 18, 2020, as Appl. No. 16/822,098.
Application 16/822,098 is a continuation of application No. PCT/US2018/051767, filed on Sep. 19, 2018.
Claims priority of provisional application 62/560,619, filed on Sep. 19, 2017.
Prior Publication US 2020/0281474 A1, Sep. 10, 2020
Int. Cl. A61B 5/00 (2006.01); A61B 5/145 (2006.01); A61B 5/1459 (2006.01); G01N 21/65 (2006.01); A61B 5/1455 (2006.01)
CPC A61B 5/0075 (2013.01) [A61B 5/1459 (2013.01); A61B 5/14546 (2013.01); A61B 5/14552 (2013.01); A61B 5/7275 (2013.01); G01N 21/65 (2013.01); A61B 2505/05 (2013.01)] 22 Claims
OG exemplary drawing
 
1. A method of monitoring a patient, the method comprising:
measuring a resonance Raman spectrum of in vivo, in situ tissue of the patient with a Raman spectroscopy system; and
quantifying a mitochondrial redox state of the in vivo, in situ tissue based on the resonance Raman spectrum,
wherein quantifying the mitochondrial redox state comprises:
performing a regression analysis of the resonance Raman spectrum using a linear combination of reference resonance Raman spectra from whole mitochondria in reduced and oxidized states, the reference resonance Raman spectra representing weighted average redox states of cytochromes in the whole mitochondria and effects of the cytochromes on each other in a mitochondrial membrane of the whole mitochondria; and
determining a redox state of whole mitochondria in the in vivo, in situ tissue based on the regression analysis of the resonance Raman spectrum.