CPC A61B 5/0075 (2013.01) [A61B 5/1459 (2013.01); A61B 5/14546 (2013.01); A61B 5/14552 (2013.01); A61B 5/7275 (2013.01); G01N 21/65 (2013.01); A61B 2505/05 (2013.01)] | 22 Claims |
1. A method of monitoring a patient, the method comprising:
measuring a resonance Raman spectrum of in vivo, in situ tissue of the patient with a Raman spectroscopy system; and
quantifying a mitochondrial redox state of the in vivo, in situ tissue based on the resonance Raman spectrum,
wherein quantifying the mitochondrial redox state comprises:
performing a regression analysis of the resonance Raman spectrum using a linear combination of reference resonance Raman spectra from whole mitochondria in reduced and oxidized states, the reference resonance Raman spectra representing weighted average redox states of cytochromes in the whole mitochondria and effects of the cytochromes on each other in a mitochondrial membrane of the whole mitochondria; and
determining a redox state of whole mitochondria in the in vivo, in situ tissue based on the regression analysis of the resonance Raman spectrum.
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