US 11,717,166 B2
Method and apparatus for the non-invasive measurement of tissue function and metabolism by determination of steady-state fluorescence anisotropy
Ralph Zuckerman, Philadelphia, PA (US)
Assigned to Cellview Imaging Inc., Toronto (CA)
Filed by Cellview Imaging Inc., Toronto (CA)
Filed on Jul. 19, 2021, as Appl. No. 17/379,227.
Application 14/974,550 is a division of application No. 13/673,377, filed on Nov. 9, 2012, granted, now 9,215,982, issued on Dec. 22, 2015.
Application 11/870,355 is a division of application No. 11/624,820, filed on Jan. 19, 2007, granted, now 8,129,105, issued on Mar. 6, 2012.
Application 17/379,227 is a continuation of application No. 16/751,678, filed on Jan. 24, 2020, granted, now 11,064,890.
Application 16/751,678 is a continuation of application No. 16/253,633, filed on Jan. 22, 2019, granted, now 10,542,891, issued on Jan. 28, 2020.
Application 16/253,633 is a continuation of application No. 16/015,659, filed on Jun. 22, 2018, granted, now 10,194,804, issued on Feb. 5, 2019.
Application 16/015,659 is a continuation of application No. 15/288,840, filed on Oct. 7, 2016, granted, now 10,004,401, issued on Jun. 26, 2018.
Application 15/288,840 is a continuation of application No. 14/974,550, filed on Dec. 18, 2015, granted, now 9,462,951, issued on Oct. 11, 2016.
Application 13/673,377 is a continuation of application No. 11/870,355, filed on Oct. 10, 2007, granted, now 8,309,346, issued on Nov. 13, 2012.
Claims priority of provisional application 60/744,831, filed on Apr. 13, 2006.
Prior Publication US 2022/0039655 A1, Feb. 10, 2022
Int. Cl. A61B 5/00 (2006.01); A61B 5/145 (2006.01); A61B 5/1455 (2006.01); G01N 21/64 (2006.01)
CPC A61B 5/0071 (2013.01) [A61B 5/1455 (2013.01); A61B 5/14546 (2013.01); A61B 5/14555 (2013.01); A61B 5/14556 (2013.01); A61B 5/4842 (2013.01); A61B 5/7246 (2013.01); A61B 5/7278 (2013.01); G01N 21/645 (2013.01); G01N 21/6445 (2013.01); G01N 2021/6484 (2013.01); G01N 2201/0683 (2013.01); G01N 2201/12 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A method of evaluating biological tissue, the method comprising:
irradiating a biological tissue with light so as to cause a fluorophor in the biological tissue to fluoresce;
determining steady-state fluorescence anisotropy of emitted fluorescence from the fluorophor;
comparing steady-state fluorescence anisotropy obtained from the biological tissue in a first state with steady-state fluorescence anisotropy obtained from the biological tissue in a second state in which the biological tissue has been subjected to a non-invasive stimulus; and
determining presence of a disease responsive to evaluating the biological tissue based on the comparison.