	US 11,716,995 B2
	Method of producing an antimicrobial agent
Durant Scholz, Lincolnton, NC (US); Tiffany Russell, Dallas, NC (US); Erica Segura, Lincolnton, NC (US); Anna Crovetto, Hamburg (DE); Maureen Danaher, Charlotte, NC (US); Monica Beltran, Fort Mill, SC (US); and Parisa Mehrzadeh, Huntersville, NC (US)
Assigned to Active Micro Technologies, LLC, Lincolnton, NC (US)
Filed by Active Micro Technologies, LLC, Lincolnton, NC (US)
Filed on Nov. 2, 2020, as Appl. No. 17/86,969.
Prior Publication US 2022/0132863 A1, May 5, 2022
Int. Cl. A01N 63/32 (2020.01); A01N 65/08 (2009.01); A01N 65/00 (2009.01)

CPC A01N 63/32 (2020.01) [A01N 65/00 (2013.01); A01N 65/08 (2013.01)]

16 Claims

1. A method for producing an antimicrobial agent, comprising

co-fermenting at least one seed of Lepidium Sativum and at least one yeast, the co-fermentation process generating a by-product comprising a yeast lees,

extracting the yeast lees,

adding the extracted yeast lees to a growth medium to form a mixture,

inoculating the mixture with at least one cell of Ganoderma Lucidum to produce an inoculated mixture comprising mycelium,

agitating the inoculated mixture, and

filtering the agitated mixture to isolate and extract the antimicrobial agent from the mycelium.