US 11,716,943 B2
Molecular markers linked to disease resistance in soybean
Ainong Shi, Fayetteville, AR (US); Harish T. Gandhi, Medchal Mandal (IN); Becky Welsh Breitinger, Research Triangle Park, NC (US); Zhanyou Xu, Slater, IA (US); Roger L. McBroom; and Harikrishnan Ramasubramaniam, Bay, AR (US)
Assigned to Syngenta Participations AG, Basel (CH)
Filed by Syngenta Participations AG, Basel (CH)
Filed on Apr. 24, 2020, as Appl. No. 16/857,252.
Application 16/857,252 is a continuation of application No. 15/000,388, filed on Jan. 19, 2016, granted, now 10,655,142.
Application 15/000,388 is a continuation of application No. 13/486,705, filed on Jun. 1, 2012, abandoned.
Claims priority of provisional application 61/492,104, filed on Jun. 1, 2011.
Prior Publication US 2020/0291418 A1, Sep. 17, 2020
Int. Cl. C12N 15/82 (2006.01); A01H 1/04 (2006.01)
CPC C12N 15/8279 (2013.01) [A01H 1/04 (2013.01)] 6 Claims
 
1. A method of producing a Glycine max plant that has improved resistance to Phytophthora as compared to a control plant, the method comprising the steps of:
(a) isolating a nucleic acid from a Glycine max plant;
(b) detecting in the nucleic acid of (a) the presence of a genetic marker that is associated with improved resistance to Phytophthora and is associated with Rps1c, wherein said genetic marker comprises SY2726DQ (SEQ ID NO: 5) and further comprises an A allele at nucleotide 251 of SEQ ID NO:5 (SY2726DQ) or is a genetic marker linked thereto, wherein said genetic marker linked thereto comprises at least one of an A allele at nucleotide 251 of SEQ ID NO: 1 (SY2723AQ), a C allele at nucleotide 251 of SEQ ID NO: 3 (SY2724AQ), a G allele at nucleotide 251 of SEQ ID NO: 4 (SY2726BQ), an A allele at nucleotide 251 of SEQ ID NO: 6 (SY2724BQ), an A allele at nucleotide 251 of SEQ ID NO: 7 (SY2724CQ), and a G allele at nucleotide 251 of SEQ ID NO: 8 (SY2725AQ);
(c) selecting a first Glycine max plant based on the presence of the marker associated with improved resistance to Phytophthora in (b);
(d) crossing a second Glycine max plant with the first Glycine max plant of (c) wherein, said second Glycine max plant does not comprise in its genome the marker associated with improved resistance to Phytophthora in (c);
(e) producing seed from the crossing of (d); and
(f) selecting a Glycine max plant, grown from the seed of (e), that has improved resistance to Phytophthora and comprises the genetic marker associated with improved resistance to Phytophthora and associated with Rps1c.