US 11,714,091 B2
Method for analyzing the 3D structure of biomolecules
Martin Andersson, Mölndal (SE); Mats Hulander, Gothenburg (SE); and Gustav Sundell, Gothenburg (SE)
Assigned to Vitroprobe Analytics AB, Gothenburg (SE)
Appl. No. 16/629,166
Filed by Martin Andersson, Mölndal (SE); Mats Hulander, Gothenburg (SE); and Gustav Sundell, Gothenburg (SE)
PCT Filed Jul. 4, 2018, PCT No. PCT/SE2018/050731
§ 371(c)(1), (2) Date Jan. 7, 2020,
PCT Pub. No. WO2019/013688, PCT Pub. Date Jan. 17, 2019.
Claims priority of application No. 1750924-1 (SE), filed on Jul. 14, 2017.
Prior Publication US 2021/0364525 A1, Nov. 25, 2021
Int. Cl. G01N 33/68 (2006.01); G01N 1/36 (2006.01); G01N 23/046 (2018.01); B81B 1/00 (2006.01)
CPC G01N 33/6803 (2013.01) [B81B 1/008 (2013.01); G01N 1/36 (2013.01); G01N 23/046 (2013.01)] 13 Claims
OG exemplary drawing
 
1. A method for determining a three dimensional (3D) structure of a biomolecule, said method comprising:
preparing (S1) an amorphous silica matrix that encapsulates said biomolecule;
preparing (S2) a needle specimen comprising said amorphous silica matrix that encapsulates said biomolecule; and
performing (S3) atom probe tomography analysis of said needle specimen to determine said 3D structure of said biomolecule,
wherein preparing (S1) said amorphous silica matrix comprises encapsulating said biomolecule via a sol-gel process that uses an organic precursor to silica and/or an inorganic precursor to silica.