US 11,714,088 B2
Early detection of colorectal cancer
Carole A. Oskeritzian, Columbia, SC (US); Franklin Berger, Columbia, SC (US); Alena P. Chumanevich, Lexington, SC (US); John W. Fuseler, Columbia, SC (US); Nabihah I. Kumte, Columbia, SC (US); and Ahmed Aladhami, Columbia, SC (US)
Assigned to Univerisy of South Carolina, Columbia, SC (US)
Filed by UNIVERSITY OF SOUTH CAROLINA, Columbia, SC (US)
Filed on Dec. 19, 2019, as Appl. No. 16/720,309.
Claims priority of provisional application 62/807,403, filed on Feb. 19, 2019.
Prior Publication US 2020/0264179 A1, Aug. 20, 2020
Int. Cl. G01N 33/574 (2006.01); A61B 10/00 (2006.01); A61B 10/02 (2006.01); G01N 1/30 (2006.01); G01N 33/50 (2006.01)
CPC G01N 33/57419 (2013.01) [A61B 10/0096 (2013.01); A61B 10/02 (2013.01); G01N 1/30 (2013.01); G01N 33/5091 (2013.01); G01N 2001/302 (2013.01)] 14 Claims
OG exemplary drawing
 
1. A method for detection of colorectal cancer or precancerous colon polyps, the method comprising:
collecting a cell sample from a rectal area of a subject;
staining the cell sample; and
examining the stained cell sample according to a computerized imaging approach for the presence or quantity of mast cells, the computerized imaging approach comprising identifying areas of the stained cell sample that include stain-positive cells, and isolating individual regions of interest in the identified areas according to a Hue, Saturation, Intensity (HSI) color model; wherein the presence of mast cells in the cell sample indicates precancerous polyps or colorectal cancer in the colon of the subject.