US 11,714,083 B2
Point-of-care apparatus and methods for analyte detections using electrochemical impedance or capacitance
Jeffrey LaBelle, Tempe, AZ (US); Garrett Repp, Tempe, AZ (US); Chi Lin, Tempe, AZ (US); Mark Spano, Casa Grande, AZ (US); Jennifer Blain Christen, Chandler, AZ (US); Hongwu Jiang, Chandler, AZ (US); Marcus Smith, Birmingham, AL (US); Andrew Penman, Birmingham, AL (US); Pierce Youngbar, Birmingham, AL (US); Mackenzie Honikel, Chandler, AZ (US); and Curtiss Cook, Scottsdale, AZ (US)
Assigned to ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY, Scottsdale, AZ (US); MAYO FOUNDATION FOR MEDICAL EDUCATION AND RESEARCH, Rochester, MN (US); and AXIM BIOTECHNOLOGIES. INC., New York, NY (US)
Appl. No. 16/612,270
Filed by Arizona Board of Regents on behalf of Arizona State University, Scottsdale, AZ (US); MAYO FOUNDATION FOR MEDICAL EDUCATION AND RESEARCH, Rochester, MN (US); and AXIM Biotechnologies, Inc., New York, NY (US)
PCT Filed May 4, 2018, PCT No. PCT/US2018/031160
§ 371(c)(1), (2) Date Nov. 8, 2019,
PCT Pub. No. WO2018/208610, PCT Pub. Date Nov. 15, 2018.
Claims priority of provisional application 62/505,004, filed on May 11, 2017.
Prior Publication US 2021/0223196 A1, Jul. 22, 2021
Int. Cl. G01N 33/543 (2006.01); G01N 27/02 (2006.01); G01N 27/327 (2006.01); G01N 33/487 (2006.01); G01N 33/53 (2006.01); G01N 33/569 (2006.01); G01N 33/66 (2006.01); G01N 35/00 (2006.01)
CPC G01N 33/5438 (2013.01) [G01N 27/026 (2013.01); G01N 27/3276 (2013.01); G01N 33/48707 (2013.01); G01N 33/5308 (2013.01); G01N 33/56983 (2013.01); G01N 33/66 (2013.01); G01N 35/00029 (2013.01); G01N 2333/075 (2013.01)] 11 Claims
OG exemplary drawing
 
1. An apparatus for detecting one or more analytes in a bodily fluid sample utilizing Electrochemical Impedance Spectroscopy (EIS) or Electrochemical Capacitance Spectroscopy (ECS), comprising:
an electrochemical sensor operably configured to provide an electrochemical impedance or electrochemical capacitance measurement of an analyte in said fluid, said sensor including a target-capturing molecule immobilized to a working electrode in a three electrode configuration, wherein the target-capturing molecule is an antibody, and wherein the antibody is physically adsorbed to the working electrode and covalently crosslinked by exposure to glutaraldehyde, thereby the antibody is directly immobilized onto the working electrode surface in dry form.