CPC G01N 30/88 (2013.01) [G01N 1/405 (2013.01); G01N 1/4077 (2013.01); G01N 2001/4088 (2013.01)] | 6 Claims |
1. A method for preparing exosomes from a biological sample, the method comprising: (i) a step for performing centrifugal separation on a biological sample to remove contaminants from the sample, and then obtaining a supernatant; (ii) a step for subjecting the supernatant obtained in the step (i) to ultrafiltration by using a tangential flow filter having a nominal molecular weight cut-off (MWCO) of about 750 kDa and a pore size of 0.22 μm, and then concentrating the supernatant; and (iii) a step for subjecting a concentrated liquid obtained in the step (ii) to anion exchange column chromatography, wherein an anion exchanger used in the anion exchange column chromatography is a weakly basic anion exchanger, which retains selectivity based on both hydrophobic interaction and hydrophobic binding formation, and has a phenyl group, an amide bond, and a carboxyl group.
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