	US 11,713,477 B2
	Methods for producing ethanol
George W. Aux, Research Triangle Park, NC (US)
Assigned to Syngenta Participations AG, Basel (CH)
Filed by Syngenta Participations AG, Basel (CH)
Filed on Aug. 24, 2021, as Appl. No. 17/410,262.
Application 17/410,262 is a division of application No. 16/874,292, filed on May 14, 2020, granted, now 11,130,978.
Application 16/519,707 is a division of application No. 15/729,011, filed on Oct. 10, 2017, granted, now 10,400,258, issued on Sep. 3, 2019.
Application 15/729,011 is a division of application No. 12/395,180, filed on Feb. 27, 2009, granted, now 9,816,119, issued on Oct. 27, 2017.
Application 16/874,292 is a continuation of application No. 16/519,707, filed on Jul. 23, 2019, granted, now 10,689,679, issued on Jun. 23, 2020.
Claims priority of provisional application 61/032,773, filed on Feb. 29, 2008.
Prior Publication US 2021/0381016 A1, Dec. 9, 2021
Int. Cl. C12P 19/14 (2006.01); C12P 7/10 (2006.01)

CPC C12P 19/14 (2013.01) [C12P 7/10 (2013.01); Y02E 50/10 (2013.01)]

14 Claims

1. A method for producing an increased amount of ethanol, the method comprising:

(a) liquefying an aqueous slurry of starch-containing plant material in the presence of at least a first and a second class of α-amylase enzymes to obtain a liquefact, wherein the first class of α-amylase enzymes exhibits a unimodal starch hydrolysis pattern and is derived from a microorganism of the order Thermococcales and the second class of α-amylase enzymes exhibits a bimodal starch hydrolysis pattern, and

(b) fermenting the liquefact to produce ethanol;

wherein the at least first and second class of α-amylase enzymes in the liquefact produces an increased amount of ethanol at 48 hours compared to a single class of enzyme.