CPC C12N 15/90 (2013.01) [C12N 9/22 (2013.01); C12N 15/1068 (2013.01); C12N 15/11 (2013.01); C12N 15/111 (2013.01); C12N 2310/20 (2017.05); C12N 2800/80 (2013.01)] | 26 Claims |
1. An engineered nuclease system comprising:
a. an endonuclease configured to be selective for a protospacer adjacent motif (PAM) sequence comprising the sequence 5′-YYn-3′ (SEQ ID NO: 3871), wherein said endonuclease is a class 2, type V Cas endonuclease, wherein said endonuclease comprises a WED II domain and a PAM-interacting domain having at least 80% amino acid sequence identity to the WED II domain and the PAM-interacting domain of the amino acid sequence of SEQ ID NO: 215; and
b. an engineered guide RNA, wherein said engineered guide RNA is configured to form a complex with said endonuclease and said engineered guide RNA comprises a spacer sequence configured to hybridize to a target nucleotide sequence.
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