US 11,713,460 B2
Protecting RNAs from degradation using engineered viral RNAs
Jeffrey S. Kieft, Denver, CO (US); Erich G. Chapman, Denver, CO (US); David A. Costantino, Denver, CO (US); Jay R. Hesselberth, Denver, CO (US); Andrea MacFadden, Denver, CO (US); and Benjamin Akiyama, Denver, CO (US)
Assigned to The Regents of the University of Colorado, Denver, CO (US)
Filed by The Regents of the University of Colorado, Denver, CO (US)
Filed on Feb. 11, 2021, as Appl. No. 17/173,354.
Application 17/173,354 is a continuation of application No. 16/064,174, granted, now 10,920,224, previously published as PCT/US2016/066723, filed on Dec. 14, 2016.
Claims priority of provisional application 62/270,986, filed on Dec. 22, 2015.
Prior Publication US 2021/0332357 A1, Oct. 28, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C07H 21/04 (2006.01); C12N 15/113 (2010.01)
CPC C12N 15/113 (2013.01) [C12N 2310/18 (2013.01); C12N 2310/531 (2013.01)] 20 Claims
 
1. A ribonucleic acid (RNA) duplex comprising a synthetic first RNA sequence that provides exonuclease resistance to the RNA duplex when hybridized to a heterologous second RNA sequence without exonuclease resistance, wherein a region of said synthetic RNA sequence and a region of said heterologous RNA sequence hybridize to form an interwoven pseudoknot structure, wherein the synthetic first RNA sequence that provides exonuclease resistance to the RNA duplex comprises a sequence of nucleotides 10-60 of SEQ ID NO:2.