US 11,713,456 B2
Isolation of nucleic acids from environmental samples using magnetic particles
Andreas von Bohl, Aachen (DE)
Assigned to AXAGARIUS GMBH & CO. KG, Düren (DE)
Filed by Axagarius GmbH & Co. KG, Dueren (DE)
Filed on Apr. 8, 2021, as Appl. No. 17/225,263.
Claims priority of application No. 20 2020 109 837.5 (DE), filed on Apr. 8, 2020.
Prior Publication US 2021/0317438 A1, Oct. 14, 2021
Int. Cl. C12N 15/10 (2006.01)
CPC C12N 15/1013 (2013.01) 35 Claims
 
1. A method for isolating nucleic acids from a biological sample or an environmental sample and depleting contaminants/humic substances from the biological sample, comprising
(a) lysing said biological sample;
(b) adding a binder solution that is free from chaotropic salts, comprising at least one organic solvent selected from the group consisting of cyclic C2-4-alkylene carbonates, C2-3-alkylene glycol diacetates, and derivatives thereof, and at least one non-ionic surfactant, and a nucleic acid-binding solid phase to the lysed sample, and separating the solid phase with the nucleic acids bound thereto;
(c) washing the separated solid phase with the nucleic acids bound thereto once or several times with the same or different washing solutions (A) and (B) that contain at least one amine compound (A) or are free of amine compounds (B), wherein at least one washing step with a washing solution (A) is performed; and
(d) desorbing the nucleic acids from the washed solid phase by adding an aqueous elution buffer.