	US 11,713,441 B2
	Cell culture substrates, methods and uses thereof
Ying-Chih Chang, Atherton, CA (US)
Assigned to Academia Sinica, Taipei (TW)
Filed by Academia Sinica, Taipei (TW)
Filed on Sep. 30, 2022, as Appl. No. 17/958,234.
Application 17/958,234 is a continuation in part of application No. 17/566,179, filed on Dec. 30, 2021, granted, now 11,492,598.
Claims priority of provisional application 63/252,268, filed on Oct. 5, 2021.
Claims priority of provisional application 63/132,934, filed on Dec. 31, 2020.
Prior Publication US 2023/0091341 A1, Mar. 23, 2023
Int. Cl. G01N 33/50 (2006.01); C12N 5/09 (2010.01); C12M 1/00 (2006.01)

CPC C12M 23/20 (2013.01) [C12N 5/0693 (2013.01); G01N 33/5011 (2013.01); G01N 33/5088 (2013.01); C12N 2502/1164 (2013.01); C12N 2513/00 (2013.01); C12N 2533/32 (2013.01); C12N 2533/40 (2013.01); C12N 2533/50 (2013.01)]

22 Claims

1. A method of preparing cell cultures, comprising:

(a) providing a cell culture substrate having a surface comprising polyelectrolyte multilayers and an absorbent polymer, wherein the absorbent polymer is in direct contact with a polycation or a polyanion of the polyelectrolyte multilayers;

(b) seeding a plurality of cancer cells on the surface, wherein the plurality of cancer cells is obtained from a fluid sample of a cancer patient, and wherein the cancer cells comprise circulating tumor cells; and

(c) culturing the cancer cells under a suitable medium for a time sufficient to produce cell cultures, wherein the cell cultures comprise three-dimensional (3D) cell cultures comprising a plurality of tumor spheroids adhered to the surface.