US 11,712,693 B2
Integrated selective capture, sequestration, fluidic isolation, electrical lysis and analysis of single cells
Robbyn K. Anand, Ames, IA (US); and Min Li, Ames, IA (US)
Assigned to Iowa State University Research Foundation, Inc., Ames, IA (US)
Filed by Iowa State University Research Foundation, Inc., Ames, IA (US)
Filed on Oct. 7, 2019, as Appl. No. 16/594,848.
Claims priority of provisional application 62/741,662, filed on Oct. 5, 2018.
Prior Publication US 2020/0114352 A1, Apr. 16, 2020
Int. Cl. B01L 3/00 (2006.01); B03C 5/00 (2006.01); C12N 13/00 (2006.01)
CPC B01L 3/502715 (2013.01) [B03C 5/005 (2013.01); C12N 13/00 (2013.01); B01L 2200/0647 (2013.01); B01L 2300/023 (2013.01); B01L 2300/0645 (2013.01); B01L 2300/12 (2013.01)] 19 Claims
OG exemplary drawing
 
1. A microfluidic device comprising:
one or more arrays of cell assay units; and
one or more fluidic microchannels that are configured to retain and move an ionically conductive solution,
wherein the one or more arrays of the cell assay units are placed along the one or more fluidic microchannels;
wherein each array of the cell assay units comprises two or more cell assay units;
wherein each of the cell assay units comprises a reaction chamber, micropocket, a leak channel, and at least one wireless bipolar electrode (BPE);
wherein the micropocket fluidically connects the reaction chamber and the one or more fluidic microchannels; and
wherein the leak channel fluidically connects the reaction chamber and the one or more fluidic microchannels.