US 11,710,627 B2
Cell population analysis
Emrys Jones, Manchester (GB); Steven Derek Pringle, Darwen (GB); Keith Richardson, High Peak (GB); James Ian Langridge, Sale (GB); and Zoltan Takats, Cambridge (GB)
Assigned to Micromass UK Limited, Wilmslow (GB)
Filed by Micromass UK Limited, Wilmslow (GB)
Filed on Dec. 22, 2021, as Appl. No. 17/558,925.
Application 17/558,925 is a continuation of application No. 15/555,694, granted, now 11,239,066, previously published as PCT/GB2016/050603, filed on Mar. 7, 2016.
Claims priority of application No. 1503863 (GB), filed on Mar. 6, 2015; application No. 1503864 (GB), filed on Mar. 6, 2015; application No. 1503867 (GB), filed on Mar. 6, 2015; application No. 1503876 (GB), filed on Mar. 6, 2015; application No. 1503877 (GB), filed on Mar. 6, 2015; application No. 1503878 (GB), filed on Mar. 6, 2015; application No. 1503879 (GB), filed on Mar. 6, 2015; application No. 1516003 (GB), filed on Sep. 9, 2015; and application No. 1518369 (GB), filed on Oct. 16, 2015.
Prior Publication US 2022/0115222 A1, Apr. 14, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 30/72 (2006.01); H01J 49/04 (2006.01); A61B 10/02 (2006.01); A61B 18/00 (2006.01); A61B 18/14 (2006.01); A61B 18/20 (2006.01); G01N 3/00 (2006.01); G01N 9/00 (2006.01); G01N 33/68 (2006.01); H01J 49/00 (2006.01); H01J 49/06 (2006.01); H01J 49/16 (2006.01); G16B 20/50 (2019.01); G01N 27/623 (2021.01); A61B 90/13 (2016.01); A61B 1/04 (2006.01); A61B 1/273 (2006.01); A61B 5/00 (2006.01); A61B 5/01 (2006.01); A61B 5/0507 (2021.01); A61B 5/055 (2006.01); A61B 6/03 (2006.01); A61B 8/13 (2006.01); A61B 10/00 (2006.01); A61B 17/00 (2006.01); A61B 18/04 (2006.01); A61B 18/18 (2006.01); A61F 13/38 (2006.01); C12Q 1/02 (2006.01); C12Q 1/04 (2006.01); C12Q 1/18 (2006.01); C12Q 1/24 (2006.01); G01N 1/22 (2006.01); G01N 27/624 (2021.01); G01N 33/487 (2006.01); G01N 33/92 (2006.01); H01J 49/02 (2006.01); H01J 49/10 (2006.01); H01J 49/14 (2006.01); H01J 49/24 (2006.01); H01J 49/26 (2006.01); G16B 20/00 (2019.01); A61B 17/32 (2006.01); G16H 10/40 (2018.01); G16H 15/00 (2018.01); G16H 50/20 (2018.01); A61B 1/00 (2006.01); A61B 1/31 (2006.01); A61B 5/145 (2006.01)
CPC H01J 49/049 (2013.01) [A61B 1/041 (2013.01); A61B 1/2736 (2013.01); A61B 5/0066 (2013.01); A61B 5/0075 (2013.01); A61B 5/015 (2013.01); A61B 5/055 (2013.01); A61B 5/0507 (2013.01); A61B 6/032 (2013.01); A61B 6/037 (2013.01); A61B 8/13 (2013.01); A61B 10/00 (2013.01); A61B 10/0041 (2013.01); A61B 10/0233 (2013.01); A61B 10/0283 (2013.01); A61B 17/00 (2013.01); A61B 18/00 (2013.01); A61B 18/04 (2013.01); A61B 18/042 (2013.01); A61B 18/14 (2013.01); A61B 18/1445 (2013.01); A61B 18/1815 (2013.01); A61B 18/20 (2013.01); A61B 90/13 (2016.02); A61F 13/38 (2013.01); C12Q 1/025 (2013.01); C12Q 1/04 (2013.01); C12Q 1/18 (2013.01); C12Q 1/24 (2013.01); G01N 1/2202 (2013.01); G01N 3/00 (2013.01); G01N 9/00 (2013.01); G01N 27/623 (2021.01); G01N 27/624 (2013.01); G01N 30/724 (2013.01); G01N 33/487 (2013.01); G01N 33/6848 (2013.01); G01N 33/6851 (2013.01); G01N 33/92 (2013.01); G16B 20/50 (2019.02); H01J 49/0004 (2013.01); H01J 49/0027 (2013.01); H01J 49/0031 (2013.01); H01J 49/0036 (2013.01); H01J 49/025 (2013.01); H01J 49/044 (2013.01); H01J 49/0404 (2013.01); H01J 49/0409 (2013.01); H01J 49/0422 (2013.01); H01J 49/0445 (2013.01); H01J 49/0459 (2013.01); H01J 49/0463 (2013.01); H01J 49/0468 (2013.01); H01J 49/061 (2013.01); H01J 49/068 (2013.01); H01J 49/10 (2013.01); H01J 49/14 (2013.01); H01J 49/16 (2013.01); H01J 49/164 (2013.01); H01J 49/24 (2013.01); H01J 49/26 (2013.01); A61B 1/00013 (2013.01); A61B 1/31 (2013.01); A61B 5/14542 (2013.01); A61B 17/320068 (2013.01); A61B 2010/0083 (2013.01); A61B 2017/320069 (2017.08); A61B 2018/00577 (2013.01); A61B 2018/00589 (2013.01); A61B 2018/00994 (2013.01); A61B 2218/002 (2013.01); A61B 2218/008 (2013.01); G01N 33/48735 (2013.01); G01N 2001/2223 (2013.01); G01N 2333/195 (2013.01); G01N 2405/00 (2013.01); G01N 2405/04 (2013.01); G01N 2405/08 (2013.01); G01N 2570/00 (2013.01); G01N 2800/26 (2013.01); G16B 20/00 (2019.02); G16H 10/40 (2018.01); G16H 15/00 (2018.01); G16H 50/20 (2018.01)] 17 Claims
OG exemplary drawing
 
1. A method of analysis using mass spectrometry and/or ion mobility spectrometry comprising:
(a) using a first device to generate smoke, aerosol or vapour from a target in vitro or ex vivo cell population and/or culture medium derived therefrom;
(b) adding a matrix to said aerosol, smoke or vapour, wherein said matrix comprises isopropanol;
(c) mass analysing and/or ion mobility analysing said smoke, aerosol or vapour, or ions derived therefrom, in order to obtain spectrometric data; and
(d) analysing said spectrometric data in order to identify and/or characterise said target in vitro or ex vivo cell population or one or more cells and/or compounds present in said target in vitro or ex vivo cell population and/or culture medium derived therefrom,
wherein the step of analysing comprises analysing said spectrometric data in order to:
analyse whether there is an infection of said in vitro or ex vivo cell population; or
analyse the genotype and/or phenotype of said in vitro or ex vivo cell population or one or more cell types present therein; or
analyse the effect of manipulating the genotype and/or phenotype of said in vitro or ex vivo cell population or one or more cell types present therein.