CPC H01J 49/049 (2013.01) [A61B 1/041 (2013.01); A61B 1/2736 (2013.01); A61B 5/0066 (2013.01); A61B 5/0075 (2013.01); A61B 5/015 (2013.01); A61B 5/055 (2013.01); A61B 5/0507 (2013.01); A61B 6/032 (2013.01); A61B 6/037 (2013.01); A61B 8/13 (2013.01); A61B 10/00 (2013.01); A61B 10/0041 (2013.01); A61B 10/0233 (2013.01); A61B 10/0283 (2013.01); A61B 17/00 (2013.01); A61B 18/00 (2013.01); A61B 18/04 (2013.01); A61B 18/042 (2013.01); A61B 18/14 (2013.01); A61B 18/1445 (2013.01); A61B 18/1815 (2013.01); A61B 18/20 (2013.01); A61B 90/13 (2016.02); A61F 13/38 (2013.01); C12Q 1/025 (2013.01); C12Q 1/04 (2013.01); C12Q 1/18 (2013.01); C12Q 1/24 (2013.01); G01N 1/2202 (2013.01); G01N 3/00 (2013.01); G01N 9/00 (2013.01); G01N 27/623 (2021.01); G01N 27/624 (2013.01); G01N 30/724 (2013.01); G01N 33/487 (2013.01); G01N 33/6848 (2013.01); G01N 33/6851 (2013.01); G01N 33/92 (2013.01); G16B 20/50 (2019.02); H01J 49/0004 (2013.01); H01J 49/0027 (2013.01); H01J 49/0031 (2013.01); H01J 49/0036 (2013.01); H01J 49/025 (2013.01); H01J 49/044 (2013.01); H01J 49/0404 (2013.01); H01J 49/0409 (2013.01); H01J 49/0422 (2013.01); H01J 49/0445 (2013.01); H01J 49/0459 (2013.01); H01J 49/0463 (2013.01); H01J 49/0468 (2013.01); H01J 49/061 (2013.01); H01J 49/068 (2013.01); H01J 49/10 (2013.01); H01J 49/14 (2013.01); H01J 49/16 (2013.01); H01J 49/164 (2013.01); H01J 49/24 (2013.01); H01J 49/26 (2013.01); A61B 1/00013 (2013.01); A61B 1/31 (2013.01); A61B 5/14542 (2013.01); A61B 17/320068 (2013.01); A61B 2010/0083 (2013.01); A61B 2017/320069 (2017.08); A61B 2018/00577 (2013.01); A61B 2018/00589 (2013.01); A61B 2018/00994 (2013.01); A61B 2218/002 (2013.01); A61B 2218/008 (2013.01); G01N 33/48735 (2013.01); G01N 2001/2223 (2013.01); G01N 2333/195 (2013.01); G01N 2405/00 (2013.01); G01N 2405/04 (2013.01); G01N 2405/08 (2013.01); G01N 2570/00 (2013.01); G01N 2800/26 (2013.01); G16B 20/00 (2019.02); G16H 10/40 (2018.01); G16H 15/00 (2018.01); G16H 50/20 (2018.01)] | 17 Claims |
1. A method of analysis using mass spectrometry and/or ion mobility spectrometry comprising:
(a) using a first device to generate smoke, aerosol or vapour from a target in vitro or ex vivo cell population and/or culture medium derived therefrom;
(b) adding a matrix to said aerosol, smoke or vapour, wherein said matrix comprises isopropanol;
(c) mass analysing and/or ion mobility analysing said smoke, aerosol or vapour, or ions derived therefrom, in order to obtain spectrometric data; and
(d) analysing said spectrometric data in order to identify and/or characterise said target in vitro or ex vivo cell population or one or more cells and/or compounds present in said target in vitro or ex vivo cell population and/or culture medium derived therefrom,
wherein the step of analysing comprises analysing said spectrometric data in order to:
analyse whether there is an infection of said in vitro or ex vivo cell population; or
analyse the genotype and/or phenotype of said in vitro or ex vivo cell population or one or more cell types present therein; or
analyse the effect of manipulating the genotype and/or phenotype of said in vitro or ex vivo cell population or one or more cell types present therein.
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