US 11,709,148 B2
Nanopore device for reversible ion and molecule sensing or migration
Nader Pourmand, San Mateo, CA (US); Boaz Vilozny, Santa Cruz, CA (US); Paolo Actis, London (GB); and R. Adam Seger, Santa Cruz, CA (US)
Assigned to The Regents of the University of California, Oakland, CA (US)
Filed by The Regents of the University of California, Oakland, CA (US)
Filed on Dec. 21, 2021, as Appl. No. 17/557,549.
Application 14/641,064 is a division of application No. 13/411,221, filed on Mar. 2, 2012, granted, now 8,980,073, issued on Mar. 17, 2015.
Application 17/557,549 is a continuation of application No. 16/422,735, filed on May 24, 2019, granted, now 11,243,188.
Application 16/422,735 is a continuation of application No. 14/641,064, filed on Mar. 6, 2015, granted, now 10,345,260, issued on Jul. 9, 2019.
Claims priority of provisional application 61/449,379, filed on Mar. 4, 2011.
Prior Publication US 2022/0229014 A1, Jul. 21, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. B01L 3/02 (2006.01); G01N 27/416 (2006.01); G01N 27/447 (2006.01); B01L 3/00 (2006.01); G01N 33/487 (2006.01); G01Q 60/44 (2010.01); G01N 27/327 (2006.01); B82Y 35/00 (2011.01)
CPC G01N 27/44791 (2013.01) [B01L 3/021 (2013.01); B01L 3/50273 (2013.01); B82Y 35/00 (2013.01); G01N 27/3277 (2013.01); G01N 27/4167 (2013.01); G01N 33/48721 (2013.01); G01Q 60/44 (2013.01); B01L 2300/0896 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A nanopipette for measuring pH of a sample, the nanopipette comprising:
(a) a capillary portion defining an interior bore of the nanopipette leading to a nanopore;
(b) the interior bore adapted for containing therein an electrode and an interior solution communicating with an exterior solution through said nanopore; and
(c) a coating on an interior surface of the nanopore, the coating comprising:
(i) a polyelectrolyte layer bound directly to the interior surface; and
(ii) a chitosan molecule linked to the polyelectrolyte layer.