US 11,709,138 B2
Multi-dimensional spectroscopy of macromolecules
David Allan Prystupa, Pinawa (CA); and John Stephen Pacak, Winnipeg (CA)
Filed by 12198681 Canada Ltd., Winnipeg (CA)
Filed on Jul. 28, 2021, as Appl. No. 17/387,553.
Claims priority of provisional application 63/059,298, filed on Jul. 31, 2020.
Prior Publication US 2022/0034817 A1, Feb. 3, 2022
Int. Cl. G01J 3/44 (2006.01); G01N 21/68 (2006.01); G01N 21/65 (2006.01); G01N 21/63 (2006.01)
CPC G01N 21/68 (2013.01) [G01N 21/65 (2013.01); G01N 2021/638 (2013.01)] 36 Claims
OG exemplary drawing
 
1. A method for obtaining information relating to a target molecule in a sample volume containing sample molecules comprising:
applying a sequence of temporally varying fields in a field direction to the sample volume caused by acoustic forces and/or by electromagnetic fields including electric fields and magnetic fields, or any combination thereof;
wherein the sequence of temporally varying fields is chosen to produce a temporal sequence of at least two different perturbed molecular configurations for said target molecule in the sample wherein the perturbed molecular configurations are at least in part correlated with the direction and temporal dependence of said applied fields;
for at least two of said perturbed molecular configurations in said temporal sequence of perturbed molecular configurations directing probe radiation incident on the sample molecules wherein at least some probe radiation with at least one wavelength in the incident probe radiation beam interacts with at least one sample molecule;
collecting interaction radiation that has at least in part interacted with at least one molecule in each of said at least two perturbed configurations;
measuring a temporal sequence of amplitudes of interaction radiation collected for one or more of directions and/or polarizations which are related to the field direction.
for each field direction and/or polarization, separating amplitudes that are related to the temporal sequence of applied fields from amplitudes that are not related to the temporal sequence of applied fields;
and obtaining information relating to a target molecule from amplitudes that are related to the temporal sequence of applied fields.