	US 11,708,614 B2
	Assays and methods for determining microbial resistance
Maria Torres-Gonzalez, Omaha, NE (US); Nancy Hanson, Omaha, NE (US); Joel Lechner, Omaha, NE (US); Stephanie Cossette, Omaha, NE (US); Cathy Scheer, Omaha, NE (US); Matthew R. Kreifels, Elkhorn, NE (US); Stacey Morrow, Omaha, NE (US); Christopher Connelly, Gretna, NE (US); Laura R. Porter, Omaha, NE (US); and Randy Fowler, Broomfield, CO (US)
Assigned to STRECK LLC, La Vista, NE (US); and CREIGHTON UNIVERSITY, Omaha, NE (US)
Appl. No. 16/310,074
Filed by STRECK, LLC, La Vista, NE (US)
PCT Filed Jun. 15, 2017, PCT No. PCT/US2017/037700 § 371(c)(1), (2) Date Dec. 14, 2018, PCT Pub. No. WO2017/218789, PCT Pub. Date Dec. 21, 2017.
Claims priority of provisional application 62/350,457, filed on Jun. 15, 2016.
Prior Publication US 2019/0330685 A1, Oct. 31, 2019
Int. Cl. C12Q 1/689 (2018.01); C12Q 1/6809 (2018.01); C12Q 1/6837 (2018.01); C12Q 1/6851 (2018.01); C12Q 1/686 (2018.01)

CPC C12Q 1/689 (2013.01) [C12Q 1/686 (2013.01); C12Q 1/6809 (2013.01); C12Q 1/6837 (2013.01); C12Q 1/6851 (2013.01); C12Q 2545/101 (2013.01); C12Q 2600/106 (2013.01); C12Q 2600/16 (2013.01); C12Q 2600/166 (2013.01)]

21 Claims

1. A kit for identification of one or more β-lactamase genes, wherein the one or more β-lactamase genes are selected from the group consisting of: CMY-2, DHA, MOX, ACC, FOX, and ACT/MIR genes, the kit comprising probes comprising the following sequences: AGGATGGCAAGGCCCACTATTTCA (SEQ ID NO: 194), AGCCATTACGTTCCAGAGTTGCGT (SEQ ID NO: 197), TCTGCTGAAGTTTRYCGAGGCMAA (SEQ ID NO: 200), TGAGACACGGTCCAGACTCCTACG (SEQ ID NO: 203), AAACCGGGCGATATGCGTCTGTAT (SEQ ID NO: 206), GATGCCATTGCYCGSGGTGAAAT (SEQ ID NO: 209), and ATGTTGGCCTGAACCCAGCG (SEQ ID NO: 212), wherein each probe comprises a fluorophore and/or a fluorescent quencher.