US 11,708,596 B2
Analyzing and using motility kinematics of microorganisms
Kwangmin Son, Cambridge, MA (US); and Roman Stocker, Boston, MA (US)
Assigned to PHAST CORP., Boston, MA (US)
Filed by PhAST Corp., Boston, MA (US)
Filed on Mar. 5, 2019, as Appl. No. 16/292,608.
Application 16/292,608 is a continuation of application No. 15/883,485, filed on Jan. 30, 2018, granted, now 10,266,867.
Claims priority of provisional application 62/453,605, filed on Feb. 2, 2017.
Prior Publication US 2019/0241928 A1, Aug. 8, 2019
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/04 (2006.01); G02B 21/00 (2006.01); G02B 21/36 (2006.01); C40B 30/06 (2006.01); C40B 40/02 (2006.01); G01N 33/94 (2006.01); G16C 10/00 (2019.01)
CPC C12Q 1/04 (2013.01) [C40B 30/06 (2013.01); C40B 40/02 (2013.01); G01N 33/9446 (2013.01); G02B 21/0084 (2013.01); G02B 21/365 (2013.01); G16C 10/00 (2019.02)] 25 Claims
 
1. A method comprising:
(a) subjecting a first portion and a second portion of a bodily sample of a human or an animal to different known concentrations of one or more antibiotics, the first portion and the second portion each containing one or more individual bacteria or fungi of one or more strains;
(b) by an imaging device, capturing one or more images of the first portion and one or more images of the second portion;
(c) by computer, measuring physical trajectories of the one or more individual bacteria or fungi from the one or more captured images of the sample first portion;
(d) by computer, generating motility kinematics data corresponding to the measured physical trajectories for at least one of the one or more individual bacteria or fungi of one or more strains in the first portion;
(e) by computer, measuring physical trajectories from the one or more captured images of the second portion;
(f) by computer, generating motility kinematics data corresponding to the measured physical trajectories for at least one of the one or more individual bacteria or fungi of one or more strains in the second portion;
(g) by computer, determining from the motility kinematics data a first physical motility of the at least one of the one or more individual bacteria or fungi of one or more strains in the first portion and a second first physical motility of the at least of the one or more individual bacteria or fungi of one or more strains in the second portion;
(h) by computer, comparing the first determined physical motility and the second determined physical motility and generating a magnitude of a difference between the first determined physical motility and the second determined physical motility; and
(i) by computer, identifying one or more strains of bacteria or fungi from the magnitude of the difference between the first determined physical motility and the second determined physical motility and from stored data indicative of relationships of physical motility of bacteria or fungi to identify one or more strains of bacteria or fungi subjected to the one or more known concentrations of antibiotics.