US 11,708,572 B2
Acoustic cell separation techniques and processes
Bart Lipkens, Bloomfield, CT (US); Kedar C. Chitale, Vernon, CT (US); Krishna N. Kumar, Wilbraham, MA (US); Walter M. Presz, Jr., Wilbraham, MA (US); Ruan Zhang, Winchester, MA (US); Benjamin Ross-Johnsrud, Northampton, MA (US); Rudolf Gilmanshin, Framingham, MA (US); Natalia Rodionova, Framingham, MA (US); and Rui Tostoes, Northampton, MA (US)
Assigned to FloDesign Sonics, Inc., West Springfield, MA (US)
Filed by FloDesign Sonics, Inc., West Springfield, MA (US)
Filed on May 23, 2019, as Appl. No. 16/421,459.
Application 16/421,459 is a continuation in part of application No. 16/010,296, filed on Jun. 15, 2018.
Application 16/010,296 is a continuation in part of application No. 15/942,316, filed on Mar. 30, 2018, granted, now 11,021,699.
Application 16/010,296 is a continuation in part of application No. 15/916,270, filed on Mar. 8, 2018, granted, now 11,377,651.
Application 16/010,296 is a continuation in part of application No. 16/421,459.
Application 16/421,459 is a continuation in part of application No. 15/788,784, filed on Oct. 19, 2017, granted, now 11,420,136.
Application 15/942,316 is a continuation in part of application No. 15/613,790, filed on Jun. 5, 2017, granted, now 10,550,382.
Application 15/942,316 is a continuation in part of application No. 16/421,459.
Application 16/421,459 is a continuation in part of application No. 15/586,116, filed on May 3, 2017, granted, now 10,640,760.
Application 15/613,790 is a continuation of application No. 15/143,481, filed on Apr. 29, 2016, granted, now 9,670,477, issued on Jun. 6, 2017.
Claims priority of provisional application 62/679,012, filed on May 31, 2018.
Claims priority of provisional application 62/675,194, filed on May 23, 2018.
Claims priority of provisional application 62/520,488, filed on Jun. 15, 2017.
Claims priority of provisional application 62/485,229, filed on Apr. 13, 2017.
Claims priority of provisional application 62/479,309, filed on Mar. 30, 2017.
Claims priority of provisional application 62/468,895, filed on Mar. 8, 2017.
Claims priority of provisional application 62/410,312, filed on Oct. 19, 2016.
Claims priority of provisional application 62/374,910, filed on Aug. 15, 2016.
Claims priority of provisional application 62/359,182, filed on Jul. 6, 2016.
Claims priority of provisional application 62/330,947, filed on May 3, 2016.
Claims priority of provisional application 62/316,933, filed on Apr. 1, 2016.
Claims priority of provisional application 62/154,690, filed on Apr. 29, 2015.
Prior Publication US 2019/0345477 A1, Nov. 14, 2019
Int. Cl. C12N 13/00 (2006.01); B01D 21/28 (2006.01); G01N 15/14 (2006.01); A61M 1/36 (2006.01); B01L 3/00 (2006.01); C12M 1/00 (2006.01); G01N 15/00 (2006.01); G01N 15/10 (2006.01)
CPC C12N 13/00 (2013.01) [A61M 1/3678 (2014.02); B01D 21/283 (2013.01); B01L 3/502761 (2013.01); C12M 47/04 (2013.01); G01N 15/1484 (2013.01); B01L 2300/0864 (2013.01); B01L 2300/0867 (2013.01); B01L 2400/0436 (2013.01); B01L 2400/0439 (2013.01); G01N 2015/0053 (2013.01); G01N 2015/1006 (2013.01); G01N 2015/149 (2013.01)] 14 Claims
 
1. A method for separating biomaterial, comprising:
applying a functionalized material to a plurality of beads that are free of ferro-magnetic material;
suspending the beads in a fluid to obtain a bead suspension;
exposing the bead suspension to biomaterial with an affinity for the functionalized material to permit the biomaterial to bind to the beads; and
flowing the bead suspension in a chamber to an acoustic field generated in the chamber that traps the beads in the chamber against fluid flow.