CPC C12N 9/1252 (2013.01) [C12P 19/34 (2013.01); C12Q 1/6806 (2013.01); C12Y 207/07007 (2013.01)] | 5 Claims |
1. An isolated recombinant DNA polymerase, which recombinant DNA polymerase comprises an amino acid sequence that is at least 80% identical to amino acids 1-340 of SEQ ID NO:1, which recombinant DNA polymerase comprises mutations at amino acid positions 42, 56, 76, 78, 79, 82, 83, 86, 152, 155, 156, 184, 189, 248, 289, 290, 291, 292, and 293, wherein identification of positions is relative to wildtype DPO4 polymerase (SEQ ID NO:1), wherein the mutations are amino acid substitutions, wherein the mutation at position 156 is selected from the group consisting of D156R, D156L, D156A, D156G, D156S, D156I, D156M, and D156T, wherein amino acids 341-352 relative to wildtype DPO4 polymerase (SEQ ID NO:1) is deleted, and which recombinant DNA polymerase exhibits polymerase activity.
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