US 11,708,566 B2
DP04 polymerase variants
Mark Stamatios Kokoris, Bothell, WA (US); Marc Prindle, Seattle, WA (US); Jack Chase, Seattle, WA (US); Robert Busam, Seattle, WA (US); Michael Kovarik, Seattle, WA (US); Salka Keller, Shoreline, WA (US); Megan Murt, Seattle, WA (US); and Greg Thiessen, Seattle, WA (US)
Assigned to Stratos Genomics, Inc., Seattle, WA (US)
Appl. No. 16/610,460
Filed by STRATOS GENOMICS INC., Seattle, WA (US)
PCT Filed May 3, 2018, PCT No. PCT/US2018/030972
§ 371(c)(1), (2) Date Nov. 1, 2019,
PCT Pub. No. WO2018/204707, PCT Pub. Date Nov. 8, 2018.
Claims priority of provisional application 62/501,526, filed on May 4, 2017.
Prior Publication US 2021/0108183 A1, Apr. 15, 2021
Int. Cl. C12N 9/12 (2006.01); C12P 19/34 (2006.01); C12Q 1/6806 (2018.01)
CPC C12N 9/1252 (2013.01) [C12P 19/34 (2013.01); C12Q 1/6806 (2013.01); C12Y 207/07007 (2013.01)] 5 Claims
 
1. An isolated recombinant DNA polymerase, which recombinant DNA polymerase comprises an amino acid sequence that is at least 80% identical to amino acids 1-340 of SEQ ID NO:1, which recombinant DNA polymerase comprises mutations at amino acid positions 42, 56, 76, 78, 79, 82, 83, 86, 152, 155, 156, 184, 189, 248, 289, 290, 291, 292, and 293, wherein identification of positions is relative to wildtype DPO4 polymerase (SEQ ID NO:1), wherein the mutations are amino acid substitutions, wherein the mutation at position 156 is selected from the group consisting of D156R, D156L, D156A, D156G, D156S, D156I, D156M, and D156T, wherein amino acids 341-352 relative to wildtype DPO4 polymerase (SEQ ID NO:1) is deleted, and which recombinant DNA polymerase exhibits polymerase activity.