US 11,708,564 B2
Microorganism expressing active D-proline reductase and method of producing active D-proline reductase
Jinseung Park, Seoul (KR); Bo Seong Park, Seoul (KR); Young Lyeol Yang, Seoul (KR); In Seok Oh, Seoul (KR); Nahum Lee, Seoul (KR); and Jun Ok Moon, Seoul (KR)
Assigned to CJ CHEILJEDANG CORPORATION, Seoul (KR)
Appl. No. 16/629,435
Filed by CJ CheilJedang Corporation, Seoul (KR)
PCT Filed Jul. 12, 2018, PCT No. PCT/KR2018/007914
§ 371(c)(1), (2) Date Jan. 8, 2020,
PCT Pub. No. WO2019/013569, PCT Pub. Date Jan. 17, 2019.
Claims priority of application No. 10-2017-0088632 (KR), filed on Jul. 12, 2017.
Prior Publication US 2021/0317419 A1, Oct. 14, 2021
Int. Cl. C12N 9/02 (2006.01); C12P 13/00 (2006.01); C12N 15/70 (2006.01)
CPC C12N 9/0004 (2013.01) [C12N 15/70 (2013.01); C12P 13/001 (2013.01); C12Y 121/04001 (2013.01)] 16 Claims
OG exemplary drawing
 
1. A microorganism comprising:
(i) a first expression vector encoding PrdA protein and a second expression vector encoding PrdH protein, or
(ii) a third expression vector encoding PrdH protein and PrdA protein, and a fourth expression vector encoding PrdB protein,
wherein the PrdH protein comprises a polypeptide having a sequence identity of at least 90% to the amino acid sequence of SEQ ID NO: 1 or SEQ ID NO: 23, the PrdA protein comprises a polypeptide having a sequence identity of at least 80% to SEQ ID NO: 3 or SEQ ID NO: 19, and the PrdB protein comprises a polypeptide having a sequence identity of at least 80% to SEQ ID NO: 5 or SEQ ID NO: 21.