	US 11,708,563 B2
	Platforms and systems for automated cell culture
Matthias Wagner, Cambridge, MA (US); Suvi Aivio, Arlington, MA (US); Mariangela Amenduni, Arlington, MA (US); Catherine Pilsmaker, Arlington, MA (US); Arnaldo Pereira, Cambridge, MA (US); Ananya Zutshi, Boston, MA (US); Anthia Toure, Boston, MA (US); Steven Nagle, Wayland, MA (US); Ozge Whiting, Pawtucket, RI (US); George Harb, Providence, RI (US); Matthew Sullivan, Westwood, MA (US); Maya Berlin-Udi, Acton, MA (US); Stefanie Morgan, Hanover, MA (US); Nick Seay, Charlottesville, VA (US); Sang Lee, Newton, MA (US); and Scott Luro, Somerville, MA (US)
Assigned to CELLINO BIOTECH, INC., Cambridge, MA (US)
Filed by Cellino Biotech, Inc., Cambridge, MA (US)
Filed on Mar. 7, 2022, as Appl. No. 17/688,857.
Claims priority of provisional application 63/311,673, filed on Feb. 18, 2022.
Claims priority of provisional application 63/298,241, filed on Jan. 11, 2022.
Claims priority of provisional application 63/297,290, filed on Jan. 7, 2022.
Claims priority of provisional application 63/295,968, filed on Jan. 3, 2022.
Claims priority of provisional application 63/288,859, filed on Dec. 13, 2021.
Claims priority of provisional application 63/284,839, filed on Dec. 1, 2021.
Claims priority of provisional application 63/282,351, filed on Nov. 23, 2021.
Claims priority of provisional application 63/249,698, filed on Sep. 29, 2021.
Claims priority of provisional application 63/239,995, filed on Sep. 2, 2021.
Claims priority of provisional application 63/226,128, filed on Jul. 27, 2021.
Claims priority of provisional application 63/222,059, filed on Jul. 15, 2021.
Claims priority of provisional application 63/216,558, filed on Jun. 30, 2021.
Claims priority of provisional application 63/210,243, filed on Jun. 14, 2021.
Claims priority of provisional application 63/196,904, filed on Jun. 4, 2021.
Claims priority of provisional application 63/194,306, filed on May 28, 2021.
Claims priority of provisional application 63/167,114, filed on Mar. 28, 2021.
Claims priority of provisional application 63/157,731, filed on Mar. 7, 2021.
Prior Publication US 2022/0284574 A1, Sep. 8, 2022
Int. Cl. G06K 9/00 (2022.01); C12N 5/074 (2010.01); C12N 5/00 (2006.01); G06T 7/00 (2017.01); C12M 1/00 (2006.01); C12M 3/00 (2006.01); C12M 1/34 (2006.01); C12M 1/36 (2006.01); C12M 1/12 (2006.01); C12M 1/06 (2006.01); C12M 1/26 (2006.01); G01N 15/10 (2006.01); B01L 3/00 (2006.01)

CPC C12N 5/0696 (2013.01) [B01L 3/502715 (2013.01); C12M 23/14 (2013.01); C12M 23/22 (2013.01); C12M 23/26 (2013.01); C12M 23/28 (2013.01); C12M 23/42 (2013.01); C12M 23/44 (2013.01); C12M 23/48 (2013.01); C12M 25/06 (2013.01); C12M 27/02 (2013.01); C12M 29/02 (2013.01); C12M 33/00 (2013.01); C12M 33/12 (2013.01); C12M 41/12 (2013.01); C12M 41/26 (2013.01); C12M 41/44 (2013.01); C12M 41/46 (2013.01); C12M 41/48 (2013.01); C12M 47/04 (2013.01); C12N 5/0081 (2013.01); G01N 15/1056 (2013.01); G06T 7/0012 (2013.01); G06T 7/0016 (2013.01); B01L 2300/0663 (2013.01); G01N 2015/1006 (2013.01); G06T 2207/10056 (2013.01); G06T 2207/10064 (2013.01); G06T 2207/20081 (2013.01); G06T 2207/30024 (2013.01); G06T 2207/30072 (2013.01)]

34 Claims

1. An imaging system, comprising:

(a) a light source configured to illuminate a cell culture sample, wherein the cell culture sample comprises one or more cells that are adherent to a surface of the cell culture chamber;

(b) an objective configured to capture light from the light source passing through the cell culture sample;

(d) an actuator coupled to the cell culture chamber or the objective, wherein the actuator is configured to perform continuous movement of (i) the cell culture chamber relative to the light source and the objective or (ii) the light source and the objective relative to the cell culture chamber, wherein the continuous movement is performed during acquisition of each of the plurality of images ; and

(e) a computer processor programmed to generate quantitative phase images of the cell culture sample based at least in part on the acquired plurality of images .