	US 11,703,514 B2
	Pregabalin immunoassays
Johnny Valdez, Fremont, CA (US); Byung Sook Moon, Palo Alto, CA (US); Alejandro A. Orozco, Gilroy, CA (US); and Gonzalo Naranjo, San Martin, CA (US)
Assigned to ARK Diagnostics, Inc., Fremont, CA (US)
Filed by ARK Diagnostics, Inc., Fremont, CA (US)
Filed on May 8, 2020, as Appl. No. 16/870,733.
Application 16/870,733 is a division of application No. 15/936,158, filed on Mar. 26, 2018, granted, now 10,690,687.
Application 15/936,158 is a division of application No. 15/045,969, filed on Feb. 17, 2016, granted, now 9,958,464, issued on May 1, 2018.
Claims priority of provisional application 62/127,689, filed on Mar. 3, 2015.
Prior Publication US 2020/0341016 A1, Oct. 29, 2020
Int. Cl. G01N 33/94 (2006.01); C07C 229/04 (2006.01); G01N 33/58 (2006.01); C07K 16/44 (2006.01); C07C 237/22 (2006.01); C12N 9/96 (2006.01); C12N 9/04 (2006.01); A61K 39/00 (2006.01)

CPC G01N 33/9406 (2013.01) [A61K 39/00 (2013.01); C07C 229/04 (2013.01); C07C 237/22 (2013.01); C07K 16/44 (2013.01); C12N 9/0006 (2013.01); C12N 9/96 (2013.01); G01N 33/581 (2013.01); C07K 2317/33 (2013.01); C12Y 101/01049 (2013.01)]

11 Claims

1. A method for detecting pregabalin, the method comprising:

combining in a reaction mixture:

(a) a sample suspected of containing pregabalin; and

(b) a conjugate comprising a compound of Formula (A):

wherein:

R¹or R²is —X—W-L-Z, wherein

when R¹is —X—W-L-Z, then R²is —NH₂,

when R²is —X—W-L-Z, then R¹is —OH, and

X is NH;

W is selected from a bond, alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, cycloalkyl, substituted cycloalkyl, heterocyclyl, substituted heterocyclyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, and carbonyl;

L is a bond or a linker; and

Z is an enzyme;

or a salt thereof, and

detecting the presence of an enzymatic reaction product of the conjugate,

wherein an increase in the enzymatic reaction product of the conjugate indicates the presence of pregabalin in the sample.