US 11,703,510 B2
Methods and products for in vivo enzyme profiling
Sangeeta N. Bhatia, Lexington, MA (US); Geoffrey A. von Maltzahn, Boston, MA (US); and Gabriel A. Kwong, Atlanta, GA (US)
Assigned to Massachusetts Institute of Technology, Cambridge, MA (US)
Filed by Massachusetts Institute of Technology, Cambridge, MA (US)
Filed on Nov. 6, 2020, as Appl. No. 17/91,075.
Application 15/966,385 is a division of application No. 14/166,481, filed on Jan. 28, 2014, granted, now 9,970,941.
Application 17/091,075 is a continuation of application No. 15/966,385, filed on Apr. 30, 2018, granted, now 10,883,998.
Application 14/166,481 is a continuation of application No. 12/715,965, filed on Mar. 2, 2010, granted, now 8,673,267.
Claims priority of provisional application 61/156,660, filed on Mar. 2, 2009.
Prior Publication US 2021/0148926 A1, May 20, 2021
Int. Cl. G01N 33/68 (2006.01); C07K 7/06 (2006.01); C12Q 1/37 (2006.01); C12Q 1/56 (2006.01)
CPC G01N 33/6848 (2013.01) [C07K 7/06 (2013.01); C12Q 1/37 (2013.01); C12Q 1/56 (2013.01); G01N 33/6842 (2013.01)] 14 Claims
 
1. A method for screening protease activity in vitro, the method comprising:
introducing, into a biological sample, a diagnostic reagent that comprises a carrier linked to a signature molecule by a peptide susceptible to cleavage by a protease with increased expression in a tumor or site of injury relative to healthy tissue;
cleaving the peptide with the protease; and
subjecting the biological sample to a multiplex analysis method in order to detect the signature molecule cleaved from the carrier to thereby screen activity of the protease in vitro.