CPC G01N 33/6848 (2013.01) [C07K 7/06 (2013.01); C12Q 1/37 (2013.01); C12Q 1/56 (2013.01); G01N 33/6842 (2013.01)] | 14 Claims |
1. A method for screening protease activity in vitro, the method comprising:
introducing, into a biological sample, a diagnostic reagent that comprises a carrier linked to a signature molecule by a peptide susceptible to cleavage by a protease with increased expression in a tumor or site of injury relative to healthy tissue;
cleaving the peptide with the protease; and
subjecting the biological sample to a multiplex analysis method in order to detect the signature molecule cleaved from the carrier to thereby screen activity of the protease in vitro.
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