US 11,703,506 B2
Systems and methods for determining a chemical state
Harvey Lee Kasdan, Jerusalem (IL); Julien Meissonnier, Jerusalem (IL); Yoav Zuta, Jerusalem (IL); Micha Rosen, Jerusalem (IL); Yael Himmel, Jerusalem (IL); Yehoshua Broder, Jerusalem (IL); Bruce Davis, Clifton, ME (US); Bruce Goldman, Jerusalem (IL); Boaz Giron, Jerusalem (IL); Zion Botesazan, Jerusalem (IL); Eliezer Blasberg, Jerusalem (IL); Ilan Semmel, Jerusalem (IL); and Jacques Aschkenasy, Jerusalem (IL)
Assigned to Accellix Ltd., Jerusalem (IL)
Filed by ACCELLIX LTD., Jerusalem (IL)
Filed on Aug. 23, 2020, as Appl. No. 17/322.
Application 17/000,322 is a continuation of application No. 16/010,520, filed on Jun. 18, 2018, granted, now 10,761,094.
Application 16/010,520 is a continuation of application No. 15/670,560, filed on Aug. 7, 2017, granted, now 10,024,855.
Application 15/670,560 is a continuation of application No. 14/646,395, granted, now 9,759,722, previously published as PCT/IL2013/000092, filed on Dec. 17, 2013.
Application 14/646,395 is a continuation in part of application No. 13/716,246, filed on Dec. 17, 2012, abandoned.
Claims priority of provisional application 61/737,854, filed on Dec. 17, 2012.
Claims priority of provisional application 61/737,856, filed on Dec. 17, 2012.
Prior Publication US 2020/0386756 A1, Dec. 10, 2020
Int. Cl. B01L 3/00 (2006.01); G01N 33/569 (2006.01); G01N 21/75 (2006.01); G01N 21/64 (2006.01); G01N 21/03 (2006.01); G01N 15/14 (2006.01); G01N 33/50 (2006.01); G01N 33/53 (2006.01); G01N 33/543 (2006.01); G01N 33/58 (2006.01); G01N 33/68 (2006.01); G01N 15/10 (2006.01); G01N 35/00 (2006.01)
CPC G01N 33/56972 (2013.01) [B01L 3/502 (2013.01); B01L 3/5027 (2013.01); B01L 3/50273 (2013.01); B01L 3/502715 (2013.01); B01L 3/502761 (2013.01); G01N 15/1459 (2013.01); G01N 15/1484 (2013.01); G01N 21/03 (2013.01); G01N 21/6428 (2013.01); G01N 21/75 (2013.01); G01N 33/5091 (2013.01); G01N 33/5302 (2013.01); G01N 33/54386 (2013.01); G01N 33/569 (2013.01); G01N 33/582 (2013.01); G01N 33/68 (2013.01); G01N 33/6872 (2013.01); B01L 2200/025 (2013.01); B01L 2200/10 (2013.01); B01L 2300/041 (2013.01); B01L 2300/0654 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0867 (2013.01); B01L 2300/0877 (2013.01); B01L 2300/0883 (2013.01); B01L 2300/123 (2013.01); B01L 2300/18 (2013.01); B01L 2400/0406 (2013.01); B01L 2400/0481 (2013.01); B01L 2400/0487 (2013.01); G01N 2015/1006 (2013.01); G01N 2021/0328 (2013.01); G01N 2021/6439 (2013.01); G01N 2021/6482 (2013.01); G01N 2035/00158 (2013.01); G01N 2333/70535 (2013.01); G01N 2333/70596 (2013.01); G01N 2800/26 (2013.01)] 21 Claims
OG exemplary drawing
 
1. A valveless, single use, microfluidic cartridge for performing an assay, the cartridge comprising:
a sealable sample chamber;
a first microfluidic blister comprising a first reagent;
optionally a second blister comprising a second reagent;
optionally a third blister comprising a third reagent;
a first mixing chamber;
a second mixing chamber;
a first bellows;
a second bellows;
a reading cuvette connected to the first mixing chamber;
wherein
the sample chamber, first blister and optionally the second blister are each connected to the first mixing chamber;
the first mixing chamber is connected to the second mixing chamber via a tortuous channel;
the first bellows is connected to the second mixing chamber;
the second bellows is adapted to force liquids from the first mixing chamber towards the reading cuvette;
and wherein the cartridge is being adapted to:
receive a sample into said sample chamber and to seal said sample chamber such that said cartridge is a closed system after sealing said sample chamber;
pass a predetermined quantity of said sample into said first mixing chamber;
transfer said first reagent from said first blister into said first mixing chamber;
operate said first bellows to transfer liquid back and forth between said first and said second mixing chambers, to mix said sample and said first reagent;
optionally transfer said second reagent from said second blister into said first mixing chamber and optionally operate said first bellows to transfer liquid back and forth between said first and said second mixing chambers;
optionally transfer said third reagent from said third blister into said second mixing chamber and optionally operate said first bellows to transfer liquid back and forth between said first and said second mixing chambers;
operate said second bellows to transfer liquid from said first mixing chamber, toward said reading cuvette.