CPC C12Q 1/6897 (2013.01) [G01N 21/6486 (2013.01)] | 14 Claims |
1. A method of identifying water from a remote water source as safe to drink or not, said method comprising:
(a) obtaining a water sample from the remote water source;
(b) contacting the water sample with a test cell system comprising a cell transfected with a library of at least 20 reporter transcription units, in which each reporter transcription unit includes a unique transcription factor-inducible promoter and produces a reporter sequence in an amount commensurate with transcription factor activity of a different stress-response pathway of the cell;
(c) conducting an assay of the test cell system to quantify a profile of activity of transcription factors in said different stress-response pathways in the cell of the test cell system;
(d) calculating, in a computer-implemented processing operation conducted at a central facility on a networked computer system including a data structure storing reference transcription factor signatures as a database library of signatures, the contamination of the water sample from the remote water source from said profile of activity of transcription factors as a cumulative index that summarizes the activities of said transcription factors, and algorithmically characterizing the contamination of the water sample from the remote water source in said computer-implemented processing operation against the reference transcription factor signatures of the database library of signatures stored in the data structure of the networked computer system, to generate a computer-implemented processing output of the contamination, comprising a water quality determination of whether water from the remote water source is safe to drink or not; and
(e) transmitting the computer-implemented processing output of the contamination, comprising the water quality determination of whether water from the remote water source is safe to drink or not, to a network of the networked computer system whereon it is accessible by a network computer user.
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