US 11,702,706 B2
Massively parallel single cell analysis
Christina Fan, San Jose, CA (US); Stephen P. A. Fodor, Palo Alto, CA (US); Glenn Fu, Dublin, CA (US); Geoffrey Richard Facer, Redwood City, CA (US); and Julie Wilhelmy, Santa Cruz, CA (US)
Assigned to Becton, Dickinson and Company, Franklin Lakes, NJ (US)
Filed by Becton, Dickinson and Company, Franklin Lakes, NJ (US)
Filed on Mar. 4, 2021, as Appl. No. 17/192,814.
Application 17/192,814 is a continuation of application No. 16/012,584, filed on Jun. 19, 2018.
Application 16/012,584 is a continuation of application No. 15/459,977, filed on Mar. 15, 2017, granted, now 10,954,570.
Application 15/459,977 is a continuation of application No. 14/872,377, filed on Oct. 1, 2015, granted, now 9,637,799, issued on May 2, 2017.
Application 14/872,377 is a continuation of application No. 14/472,363, filed on Aug. 28, 2014, granted, now 9,567,645, issued on Feb. 14, 2017.
Claims priority of provisional application 62/012,237, filed on Jun. 13, 2014.
Claims priority of provisional application 61/952,036, filed on Mar. 12, 2014.
Claims priority of provisional application 61/871,232, filed on Aug. 28, 2013.
Prior Publication US 2021/0198754 A1, Jul. 1, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/68 (2018.01); C12Q 1/6888 (2018.01); C12Q 1/6874 (2018.01); C12Q 1/6876 (2018.01); C12Q 1/6881 (2018.01)
CPC C12Q 1/6888 (2013.01) [C12Q 1/6874 (2013.01); C12Q 1/6876 (2013.01); C12Q 1/6881 (2013.01); C12Q 2600/158 (2013.01); C12Q 2600/16 (2013.01)] 26 Claims
OG exemplary drawing
 
1. An instrument system for performing multiplexed single cell assay, comprising:
a microtiter plate or a microfluidic chip; and
a plurality of particles each associated with a plurality of oligonucleotides, wherein each of the plurality of oligonucleotides comprises a cellular label sequence, a molecular label sequence, and a target-binding region, wherein the cellular label sequence of each of the plurality of oligonucleotides is the same on the same particle but different on different particles, and wherein at least 100 of the plurality of oligonucleotides associated with each of the plurality of particles comprise different molecular label sequences.