CPC C12Q 1/6876 (2013.01) [C08F 8/40 (2013.01); C12Q 1/6834 (2013.01)] | 18 Claims |
1. A method of sequencing, the method comprising:
amplifying a target polynucleotide in the presence of an oligonucleotide conjugated polymeric particle to form the polynucleotide conjugated polymeric particle, the oligonucleotide at least partially complementary to the target polynucleotide, the oligonucleotide conjugated polymeric particle formed by:
exchanging a counter ion associated with an oligonucleotide with a lipophilic counter ion to form a biomolecule complex;
dispersing the biomolecule complex in a nonaqueous solvent; and
coupling the biomolecule complex to a polymeric particle in the presence of the nonaqueous solvent to form the oligonucleotide conjugated polymeric particle;
applying the polynucleotide conjugated polymeric particle to a sequencing device;
applying a primer to the polynucleotide conjugated polymeric particle;
incorporating a nucleotide; and
detecting the incorporating.
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