US 11,702,696 B2
Conjugated polymeric particle and method of making same
Steven M. Menchen, Fremont, CA (US); Alan Blanchard, Topsfield, MA (US); Luisa Andruzzi, Beverly, MA (US); Shaheer Khan, Foster City, CA (US); Dmitriy Gremyachinskiy, San Francisco, CA (US); Alfred Lui, Sunnyvale, CA (US); Craig Stolarczyk, San Mateo, CA (US); Tanya Sokolsky, Cambridge, MA (US); and Prasanna Krishnan Thwar, San Jose, CA (US)
Assigned to LIFE TECHNOLOGIES CORPORATION, Carlsbad, CA (US)
Filed by LIFE TECHNOLOGIES CORPORATION, Carlsbad, CA (US)
Filed on Jul. 24, 2020, as Appl. No. 16/938,222.
Application 15/945,348 is a division of application No. 14/855,705, filed on Sep. 16, 2015, granted, now 9,938,577, issued on Apr. 10, 2018.
Application 16/938,222 is a continuation of application No. 15/945,348, filed on Apr. 4, 2018, granted, now 10,724,094.
Application 14/855,705 is a continuation of application No. 13/763,066, filed on Feb. 8, 2013, granted, now 9,139,667, issued on Sep. 22, 2015.
Claims priority of provisional application 61/597,064, filed on Feb. 9, 2012.
Prior Publication US 2020/0354789 A1, Nov. 12, 2020
Int. Cl. C12Q 1/6876 (2018.01); C08F 8/40 (2006.01); C12Q 1/6834 (2018.01)
CPC C12Q 1/6876 (2013.01) [C08F 8/40 (2013.01); C12Q 1/6834 (2013.01)] 18 Claims
 
1. A method of sequencing, the method comprising:
amplifying a target polynucleotide in the presence of an oligonucleotide conjugated polymeric particle to form the polynucleotide conjugated polymeric particle, the oligonucleotide at least partially complementary to the target polynucleotide, the oligonucleotide conjugated polymeric particle formed by:
exchanging a counter ion associated with an oligonucleotide with a lipophilic counter ion to form a biomolecule complex;
dispersing the biomolecule complex in a nonaqueous solvent; and
coupling the biomolecule complex to a polymeric particle in the presence of the nonaqueous solvent to form the oligonucleotide conjugated polymeric particle;
applying the polynucleotide conjugated polymeric particle to a sequencing device;
applying a primer to the polynucleotide conjugated polymeric particle;
incorporating a nucleotide; and
detecting the incorporating.