US 11,702,694 B2
Polymer coatings
Wayne N. George, Cambridge (GB); Andrew A. Brown, Cambridge (GB); Daniel Bratton, Cambridge (GB); Hongji Ren, San Diego, CA (US); and Ryan Christopher Smith, San Diego, CA (US)
Assigned to Illumina, Inc., San Diego, CA (US)
Filed by Illumina, Inc., San Diego, CA (US)
Filed on Mar. 1, 2021, as Appl. No. 17/188,109.
Application 17/188,109 is a continuation of application No. 16/380,832, filed on Apr. 10, 2019, granted, now 10,954,561.
Application 16/380,832 is a continuation of application No. 15/680,561, filed on Aug. 18, 2017, granted, now 10,266,891, issued on Apr. 23, 2019.
Application 15/680,561 is a continuation of application No. 14/679,760, filed on Apr. 6, 2015, granted, now 9,752,186, issued on Sep. 5, 2017.
Application 14/679,760 is a continuation of application No. 13/784,368, filed on Mar. 4, 2013, granted, now 9,012,022, issued on Apr. 21, 2015.
Claims priority of provisional application 61/753,833, filed on Jan. 17, 2013.
Claims priority of provisional application 61/657,508, filed on Jun. 8, 2012.
Prior Publication US 2021/0254151 A1, Aug. 19, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/6874 (2018.01); B05D 3/06 (2006.01); C09D 133/26 (2006.01); C09D 135/00 (2006.01); C12Q 1/6806 (2018.01)
CPC C12Q 1/6874 (2013.01) [B05D 3/06 (2013.01); B05D 3/065 (2013.01); C09D 133/26 (2013.01); C09D 135/00 (2013.01); C12Q 1/6806 (2013.01); Y10T 428/24802 (2015.01); Y10T 428/24917 (2015.01); Y10T 428/24926 (2015.01); Y10T 428/2998 (2015.01); Y10T 428/31536 (2015.04); Y10T 428/31935 (2015.04)] 20 Claims
 
1. A substrate comprising a first plurality of oligonucleotides and a second plurality of oligonucleotides immobilized on a surface of the substrate through a polymer that is covalently attached to the surface of the substrate, wherein the polymer comprising a recurring unit of Formula (Ia) and a recurring unit of Formula (II):

OG Complex Work Unit Chemistry
wherein:
R1 is H, alkyl, alkoxy, alkenyl, alkynyl, or optionally substituted variants thereof;
RA is azido;
each of the —(CH2)o— units is optionally substituted;
o is an integer between 1 and 50;
each R4, R4′, R5 and R5′ is independently selected from the group consisting of H, R6, OR6, —C(O)OR6, —C(O)R6, —OC(O)R6, —C(O)NR7R8, and —NR7R8;
each R6 is independently H, OH, alkyl, cycloalkyl, hydroxyalkyl, aryl, heteroaryl, or heterocyclyl, or an optionally substituted variant thereof; and
each R7 and R8 is independently H or alkyl, or R7 and R8 are joined together with the atom or atoms to which they are attached to form a heterocycle.