CPC C12Q 1/6874 (2013.01) | 18 Claims |
1. A method for determining a location of an analyte in a cell, the method comprising:
(a) separating the cell from a plurality of cells by filtering the plurality of cells through a network of microfluidic channels, wherein the cell is filtered through a microfluidic channel of the network that allows the cell to migrate towards a surface comprising an array;
(b) dispensing the cell onto the surface comprising the array, wherein the microfluidic channel of the network dispenses the cell onto a unique area of the surface, wherein the array comprises a plurality of capture probes, wherein a capture probe of the plurality of capture probes comprises: (i) a spatial barcode and (ii) a capture domain;
(c) hybridizing the analyte to the capture domain; and
(d) determining (i) all or a part of the sequence of the analyte bound to the capture domain, or a complement thereof, and (ii) the spatial barcode, or a complement thereof, and using the determined sequences of (i) and (ii) to identify the location of the analyte in the cell.
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