US 11,702,693 B2
Methods for printing cells and generating arrays of barcoded cells
Rajiv Bharadwaj, Pleasanton, CA (US)
Assigned to 10x Genomics, Inc., Pleasanton, CA (US)
Filed by 10x Genomics, Inc., Pleasanton, CA (US)
Filed on Jan. 19, 2021, as Appl. No. 17/152,276.
Claims priority of provisional application 62/964,055, filed on Jan. 21, 2020.
Prior Publication US 2021/0222241 A1, Jul. 22, 2021
Int. Cl. C12Q 1/6874 (2018.01)
CPC C12Q 1/6874 (2013.01) 18 Claims
 
1. A method for determining a location of an analyte in a cell, the method comprising:
(a) separating the cell from a plurality of cells by filtering the plurality of cells through a network of microfluidic channels, wherein the cell is filtered through a microfluidic channel of the network that allows the cell to migrate towards a surface comprising an array;
(b) dispensing the cell onto the surface comprising the array, wherein the microfluidic channel of the network dispenses the cell onto a unique area of the surface, wherein the array comprises a plurality of capture probes, wherein a capture probe of the plurality of capture probes comprises: (i) a spatial barcode and (ii) a capture domain;
(c) hybridizing the analyte to the capture domain; and
(d) determining (i) all or a part of the sequence of the analyte bound to the capture domain, or a complement thereof, and (ii) the spatial barcode, or a complement thereof, and using the determined sequences of (i) and (ii) to identify the location of the analyte in the cell.