CPC C12P 13/001 (2013.01) [C12N 1/02 (2013.01); C12N 1/20 (2013.01); C12N 9/88 (2013.01); C12N 9/96 (2013.01); C12N 11/16 (2013.01); C12N 15/52 (2013.01); C12Y 402/01001 (2013.01)] | 20 Claims |
1. A method for diamine (DA) production comprising the steps of:
a) culturing a genetically engineered microorganism in a fermenter in a medium under suitable conditions and for a sufficient period of time to form a cultured medium comprising one or more of DA carbonate, DA bicarbonate, or DA bis-bicarbonate, wherein the DA of the DA carbonate, DA bicarbonate, or DA bis-bicarbonate is selected from the group consisting of hexamethylenediamine, cadaverine, putrescine, ethylenediamine, and heptamethylenediamine, wherein carbon dioxide, carbonate, bicarbonate or carbonic acid predominantly control pH of the medium as a cultured medium, the microorganism comprising at least one exogenous nucleic acid encoding at least one enzyme of a DA synthesis pathway selected from the group consisting of a hexamethylenediamine, a cadaverine, a putrescine, an ethylenediamine, and a heptamethylenediamine synthesis pathway;
b) converting the DA carbonate, DA bicarbonate, or DA bis-bicarbonate into DA free base and carbon dioxide; and
c) isolating the DA free base.
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