US 11,702,682 B2
Method of producing and processing diamines to a diamine free base using a carbonate intermediate and an engineered microorganism
Lauri H. Suominen, San Diego, CA (US); Connor J. Galleher, San Diego, CA (US); Michael Japs, San Diego, CA (US); Mark J. Burk, San Diego, CA (US); and Cara Tracewell, San Diego, CA (US)
Assigned to Genomatica, Inc., San Diego, CA (US)
Filed by Genomatica, Inc., San Diego, CA (US)
Filed on Jul. 13, 2020, as Appl. No. 16/927,396.
Application 16/927,396 is a continuation of application No. 15/538,448, granted, now 10,711,289, previously published as PCT/US2015/067478, filed on Dec. 22, 2015.
Claims priority of provisional application 62/211,315, filed on Aug. 28, 2015.
Claims priority of provisional application 62/193,693, filed on Jul. 17, 2015.
Claims priority of provisional application 62/096,309, filed on Dec. 23, 2014.
Prior Publication US 2021/0130861 A1, May 6, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12P 13/00 (2006.01); C12N 9/88 (2006.01); C12N 1/02 (2006.01); C12N 1/20 (2006.01); C12N 9/96 (2006.01); C12N 11/16 (2006.01); C12N 15/52 (2006.01)
CPC C12P 13/001 (2013.01) [C12N 1/02 (2013.01); C12N 1/20 (2013.01); C12N 9/88 (2013.01); C12N 9/96 (2013.01); C12N 11/16 (2013.01); C12N 15/52 (2013.01); C12Y 402/01001 (2013.01)] 20 Claims
 
1. A method for diamine (DA) production comprising the steps of:
a) culturing a genetically engineered microorganism in a fermenter in a medium under suitable conditions and for a sufficient period of time to form a cultured medium comprising one or more of DA carbonate, DA bicarbonate, or DA bis-bicarbonate, wherein the DA of the DA carbonate, DA bicarbonate, or DA bis-bicarbonate is selected from the group consisting of hexamethylenediamine, cadaverine, putrescine, ethylenediamine, and heptamethylenediamine, wherein carbon dioxide, carbonate, bicarbonate or carbonic acid predominantly control pH of the medium as a cultured medium, the microorganism comprising at least one exogenous nucleic acid encoding at least one enzyme of a DA synthesis pathway selected from the group consisting of a hexamethylenediamine, a cadaverine, a putrescine, an ethylenediamine, and a heptamethylenediamine synthesis pathway;
b) converting the DA carbonate, DA bicarbonate, or DA bis-bicarbonate into DA free base and carbon dioxide; and
c) isolating the DA free base.