	US 11,702,661 B2
	Constructs for continuous monitoring of live cells
Paul Blainey, Cambridge, MA (US); Jacob Borrajo, Cambridge, MA (US); Mohamad Najia, Cambridge, MA (US); and Atray Dixit, Cambridge, MA (US)
Assigned to The Broad Institute, Inc., Cambridge, MA (US); and Massachusetts Institute of Technology, Cambridge, MA (US)
Appl. No. 16/335,512
Filed by THE BROAD INSTITUTE, INC., Cambridge, MA (US); and MASSACHUSETTS INSTITUTE OF TECHNOLOGY, Cambridge, MA (US)
PCT Filed Sep. 21, 2017, PCT No. PCT/US2017/052822 § 371(c)(1), (2) Date Mar. 21, 2019, PCT Pub. No. WO2018/057812, PCT Pub. Date Mar. 29, 2018.
Claims priority of provisional application 62/397,867, filed on Sep. 21, 2016.
Prior Publication US 2020/0017861 A1, Jan. 16, 2020
Int. Cl. C12N 15/62 (2006.01); C12Q 1/6897 (2018.01); C07H 21/04 (2006.01)

CPC C12N 15/62 (2013.01) [C07H 21/04 (2013.01); C12Q 1/6897 (2013.01); C12N 2310/20 (2017.05); C12N 2310/3519 (2013.01)]

22 Claims

1. A nucleic acid construct comprising a nucleic acid sequence encoding a fusion protein and a construct RNA sequence, the fusion protein comprising a viral export compartment protein and a construct RNA sequence capture domain, the construct RNA sequence comprising a retrieval element capable of binding the RNA sequence capture domain and a cellular polyadenylated RNA capture element, wherein expression of the fusion protein in one or more cells induces export of cellular polyadenylated RNAs bound to the cellular polyadenylated RNA capture element.