CPC C12N 15/1003 (2013.01) [B01L 3/5021 (2013.01); B01L 3/5027 (2013.01); C08F 220/286 (2020.02); C12Q 1/24 (2013.01); C12Q 1/6806 (2013.01); C08G 2261/1452 (2013.01)] | 8 Claims |
1. A method of extracting microbial cells from a blood-containing sample, the method comprising:
mixing a blood-containing sample with a blood cell lysis reagent, the blood cell lysis reagent comprising polyethylene glycol tert-octylphenyl ether and sodium polyanetholesulfonate, to obtain a mixture having a concentration of polyethylene glycol tert-octylphenyl ether between approximately 0.05 and 2.5% by volume and a concentration of sodium polyanetholesulfonate between approximately 5 and 10 mg/ml, wherein a pH of the blood cell lysis reagent is between approximately 9 and 11; and
extracting microbial cells from the mixture.
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