US 11,702,647 B2
Method for pretreatment of microbial cells
Samad Talebpour, Richmond Hill (CA); Aye Aye Khine, Thornhill (CA); Robert Maaskant, King City (CA); and Tino Alavie, Thornhill (CA)
Assigned to QVELLA CORPORATION, Richmond Hill (CA)
Filed by QVELLA CORPORATION, Richmond Hill (CA)
Filed on May 22, 2020, as Appl. No. 16/881,479.
Application 16/881,479 is a continuation of application No. 15/612,091, filed on Jun. 2, 2017, granted, now 10,662,420, issued on Jul. 18, 2017.
Application 15/612,091 is a continuation in part of application No. 14/648,161, granted, now 9,707,555, issued on Mar. 10, 2015, previously published as PCT/CA2013/000992, filed on Nov. 26, 2013.
Claims priority of provisional application 61/750,242, filed on Jan. 8, 2013.
Claims priority of provisional application 61/731,809, filed on Nov. 30, 2012.
Prior Publication US 2020/0291386 A1, Sep. 17, 2020
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/10 (2006.01); B01L 3/00 (2006.01); C12Q 1/6806 (2018.01); C12Q 1/24 (2006.01); C08F 220/28 (2006.01)
CPC C12N 15/1003 (2013.01) [B01L 3/5021 (2013.01); B01L 3/5027 (2013.01); C08F 220/286 (2020.02); C12Q 1/24 (2013.01); C12Q 1/6806 (2013.01); C08G 2261/1452 (2013.01)] 8 Claims
 
1. A method of extracting microbial cells from a blood-containing sample, the method comprising:
mixing a blood-containing sample with a blood cell lysis reagent, the blood cell lysis reagent comprising polyethylene glycol tert-octylphenyl ether and sodium polyanetholesulfonate, to obtain a mixture having a concentration of polyethylene glycol tert-octylphenyl ether between approximately 0.05 and 2.5% by volume and a concentration of sodium polyanetholesulfonate between approximately 5 and 10 mg/ml, wherein a pH of the blood cell lysis reagent is between approximately 9 and 11; and
extracting microbial cells from the mixture.