| CPC C12N 5/0663 (2013.01) [A01N 1/0221 (2013.01); A61B 17/1611 (2013.01); A61B 2017/00831 (2013.01)] | 21 Claims |
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1. A method for manipulating environmental conditions of a cadaver such that stem cells obtained from bone explanted from the cadaver comprise stem cells, among which at least 70% of recovered stem cells are viable, the method comprising steps of:
(1) identifying a human who is nearing death;
(2) recording the time of death of the human, at this time the human becomes a cadaver;
(3) placing the cadaver or an explanted bone from the cadaver into a cooling environment;
(4) recording the time when step (3) occurs;
wherein the period of time from step (2) to step (3) is referred to as the warm ischemia time (WIT) and step (3) is performed from no less than one-half to no more than about eight hours after step (2); and
(5) processing the cadaver bone for extraction of stem cells; and
(6) recording the time when step (5) begins;
wherein the period of time from step (3) to step (5) is referred to as the cold ischemia time (CIT) and step (5) is performed from no less than about seven hours to no more than about thirty-nine and one-half hours after step (3),
wherein the total ischemia time, comprising the sum of WIT and CIT, is less than about forty hours such that:
when the WIT is more than one-half hour, the CIT is no more than thirty-nine and one-half hours; and
when the WIT is about eight hours, the CIT is no more than thirty-two hours;
wherein when the sum of WIT and CIT is greater than forty hours and/or WIT is greater than eight hours and/or CIT is greater than about thirty-nine and one-half hours, fewer recovered stem cells will be viable when compared to when the sum of WIT and CIT is forty hours or less and/or WIT is from no less than about one-half to no more than about eight hours and/or CIT is from no less than about seven hours to no more than about thirty-nine and one-half hours.
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