US 11,702,630 B2
In vitro methods of differentiating stem cells into neurons and neurons generated using the same
Jessica Butts, San Francisco, CA (US); and Todd C. McDevitt, San Francisco, CA (US)
Assigned to The J. David Gladstone Institutes, a testamentary trust established under the Will of J. David Gladstone, San Francisco, CA (US); The Regents of the University of California, Oakland, CA (US); and Georgia Tech Research Corporation, Atlanta, GA (US)
Appl. No. 16/303,580
Filed by The J. David Gladstone Institutes, a testamentary trust established under the Will of J. David Gladstone, San Francisco, CA (US); Georgia Tech Research Corporation, Atlanta, GA (US); and The Regents of the University of California, Oakland, CA (US)
PCT Filed May 26, 2017, PCT No. PCT/US2017/034849
§ 371(c)(1), (2) Date Nov. 20, 2018,
PCT Pub. No. WO2017/210138, PCT Pub. Date Dec. 7, 2017.
Claims priority of provisional application 62/343,747, filed on May 31, 2016.
Prior Publication US 2020/0318065 A1, Oct. 8, 2020
Int. Cl. C12N 5/0793 (2010.01); A01K 67/027 (2006.01)
CPC C12N 5/0619 (2013.01) [A01K 67/027 (2013.01); A01K 2207/12 (2013.01); A01K 2227/105 (2013.01); A01K 2267/03 (2013.01); C12N 2501/105 (2013.01); C12N 2501/13 (2013.01); C12N 2501/15 (2013.01); C12N 2501/385 (2013.01); C12N 2501/41 (2013.01); C12N 2501/415 (2013.01); C12N 2501/42 (2013.01); C12N 2501/734 (2013.01); C12N 2506/02 (2013.01); C12N 2506/45 (2013.01); C12N 2533/90 (2013.01)] 22 Claims
 
1. A method to generate spinal cord glutamatergic interneurons from a population of human pluripotent stem cells (hPSCs) comprising,
a) culturing hPSCs in vitro in a neural induction medium comprising a retinoic acid signaling pathway activator, and
b) culturing the cells from a) in a neural induction medium comprising a retinoic acid signaling pathway activator, a Shh signaling pathway activator, and a Notch signaling pathway inhibitor,
so as to generate CHX10+ V2a interneurons.