CPC C12N 5/0619 (2013.01) [A01K 67/027 (2013.01); A01K 2207/12 (2013.01); A01K 2227/105 (2013.01); A01K 2267/03 (2013.01); C12N 2501/105 (2013.01); C12N 2501/13 (2013.01); C12N 2501/15 (2013.01); C12N 2501/385 (2013.01); C12N 2501/41 (2013.01); C12N 2501/415 (2013.01); C12N 2501/42 (2013.01); C12N 2501/734 (2013.01); C12N 2506/02 (2013.01); C12N 2506/45 (2013.01); C12N 2533/90 (2013.01)] | 22 Claims |
1. A method to generate spinal cord glutamatergic interneurons from a population of human pluripotent stem cells (hPSCs) comprising,
a) culturing hPSCs in vitro in a neural induction medium comprising a retinoic acid signaling pathway activator, and
b) culturing the cells from a) in a neural induction medium comprising a retinoic acid signaling pathway activator, a Shh signaling pathway activator, and a Notch signaling pathway inhibitor,
so as to generate CHX10+ V2a interneurons.
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