CPC C07K 16/30 (2013.01) [A61K 31/713 (2013.01); C07K 16/2821 (2013.01); C07K 16/2866 (2013.01); C07K 16/468 (2013.01); C07K 2317/31 (2013.01); C07K 2317/622 (2013.01); C07K 2317/64 (2013.01); C07K 2317/76 (2013.01); C07K 2317/77 (2013.01); C07K 2317/92 (2013.01)] | 17 Claims |
1. An engineered protein comprising an antigen binding region that binds to a guide antigen and an antigen binding region that binds to an effector antigen; wherein the guide antigen and the effector antigen are expressed on the surface of a cell; wherein the antigen density of the guide antigen is at least four-fold higher than the antigen density of the effector antigen; wherein the guide antigen is ICAM-1, EphA2, or ALCAM, and the effector antigen is LRP6; and wherein
(a) the antigen binding region that binds the guide antigen ICAM-1 comprises all six complementarity determining regions (CDRs) of SEQ ID NO: 118,
(b) the antigen binding region that binds the guide antigen EphA2 comprises all six CDRs of SEQ ID NO: 121,
(c) the antigen binding region that binds the guide antigen ALCAM comprises all six CDRs of SEQ ID NO: 106, SEQ ID NO: 109, SEQ ID NO: 112, or SEQ ID NO: 115: and
(d) the antigen binding region that binds the effector antigen LRP6 comprises all six CDRs of SEQ ID NO: 4 or SEQ ID NO: 43.
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