	US 11,702,457 B2
	Agent and method for enhancing fertility
Craig Anthony Harrison, Clayton (AU); and Kelly Louise Walton, Clayton (AU)
Assigned to Monash University, Victoria (AU)
Appl. No. 17/53,856
Filed by MONASH UNIVERSITY, Clayton (AU)
PCT Filed May 9, 2019, PCT No. PCT/AU2019/000054 § 371(c)(1), (2) Date Nov. 9, 2020, PCT Pub. No. WO2019/213690, PCT Pub. Date Nov. 14, 2019.
Claims priority of application No. 2018901579 (AU), filed on May 9, 2018.
Prior Publication US 2021/0115098 A1, Apr. 22, 2021
Int. Cl. A61K 38/00 (2006.01); C07K 14/475 (2006.01); C12N 5/075 (2010.01)

CPC C07K 14/475 (2013.01) [C12N 5/0609 (2013.01); A61K 38/00 (2013.01); C12N 2501/19 (2013.01)]

19 Claims

1. An agent for promoting proliferation and differentiation of granulosa cells, said agent comprising a growth and differentiation factor-9 (GDF9) protein comprising a modified GDF9 polypeptide monomer which includes at least one amino acid substitution that enhances binding to and/or activation of activin-like kinase 4 and/or 5 receptor (ALK4/5), wherein the at least one amino acid substitution that enhances binding to and/or activation of ALK4/5 is at an amino acid position selected from the group consisting of positions 363, 366 and 369 from the human wild-type sequence or corresponding positions of other GDF9 proteins.