US 11,702,447 B2
Methods for producing collagen
Ali Elnajjar, Sandy Springs, GA (US); Ali Mourad, Duluth, GA (US); Mark Ernst Brandt, Terre Haute, IN (US); and Christopher Lippelt, Greenwood, IN (US)
Assigned to AVICENNA NUTRACEUTICAL, INC., Alpharetta, GA (US)
Appl. No. 16/611,937
Filed by AVICENNA NUTRACEUTICAL, LLC, Alpharetta, GA (US)
PCT Filed May 10, 2018, PCT No. PCT/US2018/031941
§ 371(c)(1), (2) Date Nov. 8, 2019,
PCT Pub. No. WO2018/209008, PCT Pub. Date Nov. 15, 2018.
Claims priority of provisional application 62/504,632, filed on May 11, 2017.
Prior Publication US 2021/0079038 A1, Mar. 18, 2021
Int. Cl. C07K 1/30 (2006.01); C07K 14/78 (2006.01)
CPC C07K 1/30 (2013.01) [C07K 14/78 (2013.01); C12Y 304/23001 (2013.01)] 38 Claims
 
1. A method for isolating collagen from a collagen source, the method comprising
(a) contacting the collagen source with an aqueous base to produce a first composition comprising a first supernatant and a first precipitate, wherein the aqueous base comprises an alkali base or an alkaline earth metal base, wherein the alkali base or the alkaline earth metal base has a concentration of 0.05M to 0.5M;
(b) separating the first precipitate from the first supernatant;
(c) contacting the first precipitate with a digestive enzyme at a temperature greater than or equal to 25° C. to produce a second composition comprising a second supernatant and a second precipitate, wherein the digestive enzyme comprises protease, papain, bromelain, chymotrypsin, rennin, elastase, pancreatic amylase, pancreatic lipase, pepsin, trypsin, or any combination thereof;
(d) separating the second supernatant from the second precipitate;
(e) deactivating the digestion enzyme in the second supernatant comprising adjusting the pH of the second supernatant to a pH greater than 7.0 but less than or equal to 11 to produce a third composition;
(f) precipitating the collagen from the third composition; and
(g) separating the precipitated collagen from the third composition.