CPC C07K 1/30 (2013.01) [C07K 14/78 (2013.01); C12Y 304/23001 (2013.01)] | 38 Claims |
1. A method for isolating collagen from a collagen source, the method comprising
(a) contacting the collagen source with an aqueous base to produce a first composition comprising a first supernatant and a first precipitate, wherein the aqueous base comprises an alkali base or an alkaline earth metal base, wherein the alkali base or the alkaline earth metal base has a concentration of 0.05M to 0.5M;
(b) separating the first precipitate from the first supernatant;
(c) contacting the first precipitate with a digestive enzyme at a temperature greater than or equal to 25° C. to produce a second composition comprising a second supernatant and a second precipitate, wherein the digestive enzyme comprises protease, papain, bromelain, chymotrypsin, rennin, elastase, pancreatic amylase, pancreatic lipase, pepsin, trypsin, or any combination thereof;
(d) separating the second supernatant from the second precipitate;
(e) deactivating the digestion enzyme in the second supernatant comprising adjusting the pH of the second supernatant to a pH greater than 7.0 but less than or equal to 11 to produce a third composition;
(f) precipitating the collagen from the third composition; and
(g) separating the precipitated collagen from the third composition.
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