US 11,701,655 B2
Device for analysis of cellular motility
Jacob Mollenbach Larsen, Copenhagen (DK); and Steen Broch Laursen, Odense (DK)
Assigned to MOTILITYCOUNT APS, Copenhagen (DK)
Filed by MOTILITYCOUNT APS, Copenhagen NV (DK)
Filed on Jul. 15, 2020, as Appl. No. 16/929,791.
Application 16/929,791 is a continuation of application No. 14/888,700, abandoned, previously published as PCT/DK2014/050117, filed on May 2, 2014.
Claims priority of application No. PA201370251 (DK), filed on May 3, 2013.
Prior Publication US 2021/0001335 A1, Jan. 7, 2021
Int. Cl. G01N 1/40 (2006.01); B01L 3/00 (2006.01); G01N 15/00 (2006.01); C12M 1/34 (2006.01)
CPC B01L 3/502753 (2013.01) [G01N 1/4077 (2013.01); B01L 2300/04 (2013.01); B01L 2300/0681 (2013.01); B01L 2300/087 (2013.01); B01L 2400/0478 (2013.01); B01L 2400/0487 (2013.01); C12M 41/46 (2013.01); G01N 2001/4088 (2013.01); G01N 2015/0003 (2013.01); G01N 2015/0065 (2013.01)] 11 Claims
 
1. A mesoscale fluidic system for separating motile cells from non-motile cells from a sample containing motile cells and non-motile cells, the system comprising a sub-strate having a sample chamber and an analysis chamber;
the sample chamber comprising a cell permeable filter having a pore size in the range of 1 μm to 20 μm and defining a sample application compartment and a conditioning medium compartment positioned below and above, respectively, the cell permeable filter with reference to the gravitational field,
which sample application compartment is below the cell permeable filter, and which conditioning medium compartment is above the cell permeable filter, the sample application compartment having a sample inlet port, and the conditioning medium compartment having a medium inlet port in fluid communication with the ambient environment so that
when conditioning medium is withdrawn from the conditioning medium compartment through the entry port of the analysis chamber the pressure is relieved by the medium inlet port, thereby preventing suction of cells from the sample application chamber through the cell permeable filter
and
the analysis chamber being in fluid communication with the conditioning medium compartment and the ambient environment.