	US 11,701,392 B2
	Compositions for establishing mixed chimerism and methods of manufacture thereof
Steven R. Deitcher, San Mateo, CA (US)
Assigned to MEDEOR THERAPEUTICS, INC., South San Francisco, CA (US)
Filed by MEDEOR THERAPEUTICS, INC., South San Francisco, CA (US)
Filed on Nov. 25, 2020, as Appl. No. 17/104,011.
Application 17/104,011 is a continuation of application No. 15/946,099, filed on Apr. 5, 2018, granted, now 10,881,692.
Prior Publication US 2021/0077534 A1, Mar. 18, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. A61K 35/28 (2015.01)

CPC A61K 35/28 (2013.01)

20 Claims

1. A method of manufacturing a cellular product for establishing mixed chimerism in a solid organ transplant recipient, the method comprising:

receiving an apheresis product containing CD34⁺ cells and CD3⁺ cells from a solid organ transplant donor;

dividing the apheresis product into a first portion and a second portion;

purifying the first portion to obtain an enriched amount of CD34⁺ cells;

introducing from the second portion to the enriched amount of CD34⁺ cells

(i) the obtained CD3⁺ cells in an amount greater than 1×10⁵CD3⁺ cells/kg recipient weight and

ii) circulating factors from blood of the donor, thereby creating a cellular product for establishing mixed chimerism in a solid organ transplant recipient.