US 11,693,016 B2
Systems and methods for electrochemical point-of-care detection of hemoglobin
Donnie Smith, Indianapolis, IN (US); Brittney L. Werner, Fairland, IN (US); Gary L. Hughes, Camby, IN (US); and Jie Chen, Whitestown, IN (US)
Assigned to Polymer Technology Systems, Inc., Whitestown, IN (US)
Filed by POLYMER TECHNOLOGY SYSTEMS, INC., Whitestown, IN (US)
Filed on Nov. 27, 2019, as Appl. No. 16/698,872.
Claims priority of provisional application 62/772,789, filed on Nov. 29, 2018.
Prior Publication US 2020/0174024 A1, Jun. 4, 2020
Int. Cl. G01N 33/72 (2006.01); G01N 27/327 (2006.01); C12N 9/50 (2006.01)
CPC G01N 33/721 (2013.01) [C12N 9/50 (2013.01); G01N 27/3272 (2013.01); G01N 33/723 (2013.01)] 15 Claims
OG exemplary drawing
 
1. A method for electrochemically detecting free hemoglobin in a sample solution comprising:
oxidizing the free hemoglobin;
cleaving fructosyl-valine-histidine (FVH) from the free hemoglobin to form an electrochemical test solution comprising a mixture of hemoglobin, FVH, and hemoglobin degradation products;
reacting a first portion of the electrochemical test solution with a chemistry including fructosyl peptide oxidase and a reduced ruthenium mediator to form a first reaction product;
measuring a first electrical property of the first reaction product which is indicative of an amount of hemoglobin A1C in the sample solution;
reacting a second portion of the electrochemical test solution with a chemistry including fructosyl peptide oxidase and ferrocyanide to form a second reaction product;
measuring a second electrical property of the second reaction product which is indicative of an amount of total hemoglobin in the sample solution; and
determining the amounts of hemoglobin A1C and of total hemoglobin of the sample solution based on the measured first and second electrical properties, respectively.