CPC G01N 33/721 (2013.01) [C12N 9/50 (2013.01); G01N 27/3272 (2013.01); G01N 33/723 (2013.01)] | 15 Claims |
1. A method for electrochemically detecting free hemoglobin in a sample solution comprising:
oxidizing the free hemoglobin;
cleaving fructosyl-valine-histidine (FVH) from the free hemoglobin to form an electrochemical test solution comprising a mixture of hemoglobin, FVH, and hemoglobin degradation products;
reacting a first portion of the electrochemical test solution with a chemistry including fructosyl peptide oxidase and a reduced ruthenium mediator to form a first reaction product;
measuring a first electrical property of the first reaction product which is indicative of an amount of hemoglobin A1C in the sample solution;
reacting a second portion of the electrochemical test solution with a chemistry including fructosyl peptide oxidase and ferrocyanide to form a second reaction product;
measuring a second electrical property of the second reaction product which is indicative of an amount of total hemoglobin in the sample solution; and
determining the amounts of hemoglobin A1C and of total hemoglobin of the sample solution based on the measured first and second electrical properties, respectively.
|