US 11,692,978 B2
VOC markers in saliva for diagnosis of gastric cancer and gastric cancer diagnostic method using same
Daxiang Cui, Shanghai (CN); Cuili Xue, Shanghai (CN); and Yunsheng Chen, Shanghai (CN)
Assigned to Shanghai Jiao Tong University, Shanghai (CN)
Filed by Shanghai Jiao Tong University, Shanghai (CN)
Filed on Apr. 30, 2020, as Appl. No. 16/862,617.
Claims priority of application No. 201910362235.8 (CN), filed on Apr. 30, 2019.
Prior Publication US 2020/0348269 A1, Nov. 5, 2020
Int. Cl. G01N 30/06 (2006.01); G01N 1/22 (2006.01); G01N 30/72 (2006.01); A61B 10/00 (2006.01); A61B 5/08 (2006.01)
CPC G01N 30/06 (2013.01) [A61B 10/0051 (2013.01); G01N 1/2226 (2013.01); G01N 30/7206 (2013.01); A61B 5/082 (2013.01); G01N 2001/2229 (2013.01)] 2 Claims
OG exemplary drawing
 
1. A method of diagnosing gastric cancer in a subject, the method comprising steps of:
a) obtaining a saliva sample from the subject;
b) detecting volatile organic compound (VOC) markers in the saliva sample by:
concentrating the VOC markers in the saliva sample using a solid-phase microextraction device; and
performing an analysis using a gas chromatography-mass spectrometry under a temperature program of 35° C. for 10 minutes (min), rise to 200° C. at a rate of 8° C./min, rise to 220° C. at a rate of 15° C./min and 220° C. for 12 min,
wherein the VOC markers are compounds selected from the group consisting of acetaldehyde, isopropanol, hexanal, n-butanol, nonanal, menthone, menthol, anethole, dodecanol, and a combination thereof; and
c) diagnosing the subject with gastric cancer when at least three of the compounds of acetaldehyde, isopropanol, hexanal, n-butanol and nonanal are present and at least three of the compounds of menthone, menthol, anethole and dodecanol are absent in the saliva sample.