CPC G01N 30/06 (2013.01) [A61B 10/0051 (2013.01); G01N 1/2226 (2013.01); G01N 30/7206 (2013.01); A61B 5/082 (2013.01); G01N 2001/2229 (2013.01)] | 2 Claims |
1. A method of diagnosing gastric cancer in a subject, the method comprising steps of:
a) obtaining a saliva sample from the subject;
b) detecting volatile organic compound (VOC) markers in the saliva sample by:
concentrating the VOC markers in the saliva sample using a solid-phase microextraction device; and
performing an analysis using a gas chromatography-mass spectrometry under a temperature program of 35° C. for 10 minutes (min), rise to 200° C. at a rate of 8° C./min, rise to 220° C. at a rate of 15° C./min and 220° C. for 12 min,
wherein the VOC markers are compounds selected from the group consisting of acetaldehyde, isopropanol, hexanal, n-butanol, nonanal, menthone, menthol, anethole, dodecanol, and a combination thereof; and
c) diagnosing the subject with gastric cancer when at least three of the compounds of acetaldehyde, isopropanol, hexanal, n-butanol and nonanal are present and at least three of the compounds of menthone, menthol, anethole and dodecanol are absent in the saliva sample.
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